TY - JOUR
T1 - Entorhinal Layer II Calbindin-Expressing Neurons Originate Widespread Telencephalic and Intrinsic Projections
AU - Ohara, Shinya
AU - Gianatti, Michele
AU - Itou, Kazuki
AU - Berndtsson, Christin H.
AU - Doan, Thanh P.
AU - Kitanishi, Takuma
AU - Mizuseki, Kenji
AU - Iijima, Toshio
AU - Tsutsui, Ken Ichiro
AU - Witter, Menno P.
N1 - Funding Information:
We thank Grethe M. Olsen, Paulo JB. Girão, and Bruno Monterotti for help with histological preparations and digital microscopic imaging. Funding. This work has been supported by the Kavli Foundation, the Centre of Excellence scheme – Centre for Neural Computation and research grant # 227769 of the Research Council of Norway, The Egil and Pauline Braathen and Fred Kavli Centre for Cortical Microcircuits, and the National Infrastructure scheme of the Research Council of Norway – NORBRAIN #197467. This work has also been supported by Grants-in-Aid for Scientific Research on Innovative Areas (#16H01495), and by Grant-in-Aid for Scientific Research (KAKENHI, #15K18358, #19K06917, and #16H04656) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan.
Publisher Copyright:
© Copyright © 2019 Ohara, Gianatti, Itou, Berndtsson, Doan, Kitanishi, Mizuseki, Iijima, Tsutsui and Witter.
PY - 2019/10/15
Y1 - 2019/10/15
N2 - In the present study we provide the first systematic and quantitative hodological study of the calbindin-expressing (CB+) principal neurons in layer II of the entorhinal cortex and compared the respective projections of the lateral and medial subdivisions of the entorhinal cortex. Using elaborate quantitative retrograde tracing, complemented by anterograde tracing, we report that the layer II CB+ population comprises neurons with diverse, mainly excitatory projections. At least half of them originate local intrinsic and commissural projections which distribute mainly to layer I and II. We further show that long-range CB+ projections from the two entorhinal subdivisions differ substantially in that MEC projections mainly target field CA1 of the hippocampus, whereas LEC CB+ projections distribute much more widely to a substantial number of known forebrain targets. This connectional difference between the CB+ populations in LEC and MEC is reminiscent of the overall projection pattern of the two entorhinal subdivisions.
AB - In the present study we provide the first systematic and quantitative hodological study of the calbindin-expressing (CB+) principal neurons in layer II of the entorhinal cortex and compared the respective projections of the lateral and medial subdivisions of the entorhinal cortex. Using elaborate quantitative retrograde tracing, complemented by anterograde tracing, we report that the layer II CB+ population comprises neurons with diverse, mainly excitatory projections. At least half of them originate local intrinsic and commissural projections which distribute mainly to layer I and II. We further show that long-range CB+ projections from the two entorhinal subdivisions differ substantially in that MEC projections mainly target field CA1 of the hippocampus, whereas LEC CB+ projections distribute much more widely to a substantial number of known forebrain targets. This connectional difference between the CB+ populations in LEC and MEC is reminiscent of the overall projection pattern of the two entorhinal subdivisions.
KW - commissural projections
KW - connectivity
KW - lateral entorhinal cortex
KW - long-range intrinsic projections
KW - medial entorhinal cortex
KW - parahippocampus
KW - rodent
UR - http://www.scopus.com/inward/record.url?scp=85074353764&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85074353764&partnerID=8YFLogxK
U2 - 10.3389/fnsys.2019.00054
DO - 10.3389/fnsys.2019.00054
M3 - Article
AN - SCOPUS:85074353764
SN - 1662-5137
VL - 13
JO - Frontiers in Systems Neuroscience
JF - Frontiers in Systems Neuroscience
M1 - 54
ER -