Enzyme Immunoassay for Ursodeoxycholic Acid 7-N-Acetylglucosaminides in Human Urine

Toshifumi Niwa; Kazuhiro Fujita; Toko Kasuya; Shigeo Ikegawa; Junichi Goto

doi:10.2116/analsci.12.565

Enzyme Immunoassay for Ursodeoxycholic Acid 7-N-Acetylglucosaminides in Human Urine

Toshifumi Niwa, Kazuhiro Fujita, Toko Kasuya, Shigeo Ikegawa, Junichi Goto

Research output: Contribution to journal › Article › peer-review

Abstract

A specific enzyme immunoassay (EIA) for ursodeoxycholic acid 7-N-acetylglucosaminides (UDCA-7-NAGs), novel metabolites of ursodeoxycholic acid, has been developed. The EIA was based upon antisera elicited against the glycine conjugated UDCA-7-NAG-bovine serum albumin conjugate and horseradish peroxidase-labeled-UDCA-7-NAG. The sensitivity was significantly improved by the utilization of the bridge heterologous combination system. An appropriate dose response curve of EIA for glycine conjugated UDCA-7-NAG was obtained in the range of 0.3-6ng/tube. The specificity of EIA proved to be satisfactory in terms of cross-reactivities to related bile acids. The proposed method is useful for the determination of UDCA-7-NAGs in human urine with acceptable reliability.

Original language	English
Pages (from-to)	565-568
Number of pages	4
Journal	analytical sciences
Volume	12
Issue number	4
DOIs	https://doi.org/10.2116/analsci.12.565
Publication status	Published - 1996
Externally published	Yes

Keywords

Bile acid
Enzyme immunoassay
Horseradish peroxidase
Ursodeoxycholic acid 7-N-acetylglucosaminide

ASJC Scopus subject areas

Analytical Chemistry

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title = "Enzyme Immunoassay for Ursodeoxycholic Acid 7-N-Acetylglucosaminides in Human Urine",

abstract = "A specific enzyme immunoassay (EIA) for ursodeoxycholic acid 7-N-acetylglucosaminides (UDCA-7-NAGs), novel metabolites of ursodeoxycholic acid, has been developed. The EIA was based upon antisera elicited against the glycine conjugated UDCA-7-NAG-bovine serum albumin conjugate and horseradish peroxidase-labeled-UDCA-7-NAG. The sensitivity was significantly improved by the utilization of the bridge heterologous combination system. An appropriate dose response curve of EIA for glycine conjugated UDCA-7-NAG was obtained in the range of 0.3-6ng/tube. The specificity of EIA proved to be satisfactory in terms of cross-reactivities to related bile acids. The proposed method is useful for the determination of UDCA-7-NAGs in human urine with acceptable reliability.",

keywords = "Bile acid, Enzyme immunoassay, Horseradish peroxidase, Ursodeoxycholic acid 7-N-acetylglucosaminide",

author = "Toshifumi Niwa and Kazuhiro Fujita and Toko Kasuya and Shigeo Ikegawa and Junichi Goto",

year = "1996",

doi = "10.2116/analsci.12.565",

language = "English",

volume = "12",

pages = "565--568",

journal = "Analytical Sciences",

issn = "0910-6340",

publisher = "Japan Society for Analytical Chemistry",

number = "4",

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TY - JOUR

T1 - Enzyme Immunoassay for Ursodeoxycholic Acid 7-N-Acetylglucosaminides in Human Urine

AU - Niwa, Toshifumi

AU - Fujita, Kazuhiro

AU - Kasuya, Toko

AU - Ikegawa, Shigeo

AU - Goto, Junichi

PY - 1996

Y1 - 1996

N2 - A specific enzyme immunoassay (EIA) for ursodeoxycholic acid 7-N-acetylglucosaminides (UDCA-7-NAGs), novel metabolites of ursodeoxycholic acid, has been developed. The EIA was based upon antisera elicited against the glycine conjugated UDCA-7-NAG-bovine serum albumin conjugate and horseradish peroxidase-labeled-UDCA-7-NAG. The sensitivity was significantly improved by the utilization of the bridge heterologous combination system. An appropriate dose response curve of EIA for glycine conjugated UDCA-7-NAG was obtained in the range of 0.3-6ng/tube. The specificity of EIA proved to be satisfactory in terms of cross-reactivities to related bile acids. The proposed method is useful for the determination of UDCA-7-NAGs in human urine with acceptable reliability.

AB - A specific enzyme immunoassay (EIA) for ursodeoxycholic acid 7-N-acetylglucosaminides (UDCA-7-NAGs), novel metabolites of ursodeoxycholic acid, has been developed. The EIA was based upon antisera elicited against the glycine conjugated UDCA-7-NAG-bovine serum albumin conjugate and horseradish peroxidase-labeled-UDCA-7-NAG. The sensitivity was significantly improved by the utilization of the bridge heterologous combination system. An appropriate dose response curve of EIA for glycine conjugated UDCA-7-NAG was obtained in the range of 0.3-6ng/tube. The specificity of EIA proved to be satisfactory in terms of cross-reactivities to related bile acids. The proposed method is useful for the determination of UDCA-7-NAGs in human urine with acceptable reliability.

KW - Bile acid

KW - Enzyme immunoassay

KW - Horseradish peroxidase

KW - Ursodeoxycholic acid 7-N-acetylglucosaminide

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DO - 10.2116/analsci.12.565

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VL - 12

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JO - Analytical Sciences

JF - Analytical Sciences

IS - 4

ER -

Enzyme Immunoassay for Ursodeoxycholic Acid 7-N-Acetylglucosaminides in Human Urine

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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