Abstract
Enzyme labeling of a steroid by the activated ester method has been investigated. The reaction of the N-hydroxysuccinimide ester of a testosterone derivative with β-D-galactosidase provided a conjugate suitable for enzyme immunoassay. Dose-response curves with satisfactory sensitivity and range of measurement could be obtained by the use of conjugates prepared at a limited molar ratio of steroid to enzyme. The activated ester method proved to be useful for enzyme labeling of antigen because of its simplicity and excellent reproducibility.
| Original language | English |
|---|---|
| Pages (from-to) | 2147-2150 |
| Number of pages | 4 |
| Journal | Chemical and Pharmaceutical Bulletin |
| Volume | 27 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 1979 |
Keywords
- N-hydroxysuccinimide ester
- activated ester method
- dose-response curve
- double antibody method
- enzyme immunoassay
- enzyme labeling of steroid
- immunoreactivity
- molar ratio of steroid to enzyme
- sensitivity
- testosterone-β-D-galactosidase conjugate
ASJC Scopus subject areas
- Chemistry(all)
- Drug Discovery