Enzyme Labeling of Steroids by the Activated Ester Method

Hiroshi Hosoda, Yasuo Sakai, Hiroko Yoshida, Toshio Nambara

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)


Enzyme labeling of a steroid by the activated ester method has been investigated. The reaction of the N-hydroxysuccinimide ester of a testosterone derivative with β-D-galactosidase provided a conjugate suitable for enzyme immunoassay. Dose-response curves with satisfactory sensitivity and range of measurement could be obtained by the use of conjugates prepared at a limited molar ratio of steroid to enzyme. The activated ester method proved to be useful for enzyme labeling of antigen because of its simplicity and excellent reproducibility.

Original languageEnglish
Pages (from-to)2147-2150
Number of pages4
JournalChemical and Pharmaceutical Bulletin
Issue number9
Publication statusPublished - 1979


  • N-hydroxysuccinimide ester
  • activated ester method
  • dose-response curve
  • double antibody method
  • enzyme immunoassay
  • enzyme labeling of steroid
  • immunoreactivity
  • molar ratio of steroid to enzyme
  • sensitivity
  • testosterone-β-D-galactosidase conjugate

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery


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