Enzyme printed enzyme electrode sensor for low concentration lactate monitoring

In this research, enzyme electrode sensor for measuring low concentration lactate was fabricated. In our previous work, microperfusion needle to measure concentration of lactate in subepidermal tissue have been developed. Using this microperfusion needle, lactate concentration of perfusate was about one percent of lactate concentration of blood. In order to measure this low concentration of lactate, enzyme electrode sensor using printed enzyme was fabricated. Au electrodes were patterned on the glass substrate. Ag nanometal ink was printed on reference electrode. Os-HRP polymer was printed by pasting needle type printer and lactate oxidase was printed by inkjet printer on working electrode. Addition agents which do not inactivate enzymes as wetting agent and viscosity modifier were selected. A flow channel was pasted on fabricated electrodes to measure concentration changes continuously. Fabricated sensor had high sensitivity and could measure low concentration lactate (0.01-0.25 mM). In addition, fabricated sensor was not affected by interfering substances.