Abstract
In recent years, cytosine methylation in mammalian mitochondrial DNA is one of the intensely studied topics in mitochondrial biology and medicine. It could profoundly impact gene expression, replication and maintenance of mitochondrial DNA, and abnormalities in mitochondrial DNA methylation might cause diseases with mitochondrial dysfunction. Therefore, elucidating whether mitochondrial DNA is methylated or not is of pivotal importance. However, despite a number of studies suggesting the occurrence of mitochondrial DNA methylation, it appears that no conclusive landscape or role of the methylation comes to consensus. On the other hand, our recent investigation revealed challenging points in studying methylation on mitochondrial DNA. This chapter will discuss these points and propose accurate estimation of the status of 5-methylcytosine in mitochondrial DNA.
| Original language | English |
|---|---|
| Title of host publication | The Human Mitochondrial Genome |
| Subtitle of host publication | From Basic Biology to Disease |
| Publisher | Elsevier |
| Pages | 71-85 |
| Number of pages | 15 |
| ISBN (Electronic) | 9780128196564 |
| DOIs | |
| Publication status | Published - 2020 Jan 1 |
| Externally published | Yes |
Keywords
- 5-methylcytosine
- 5mC
- Bisulfite sequencing
- DNA methyltransferase
- DNMT
- Mass spectrometry
- McrBC
- Methylation
- Mitochondrial DNA
- mtDNA
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)