Epitope Mapping of an Anti-CD10 Monoclonal Antibody (MME/1870) Using Enzyme-Linked Immunosorbent Assay

Hiroki Kawabata, Hiroyuki Suzuki, Junko Takei, Mika K. Kaneko, Yukinari Kato

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


CD10 is a glycosylated transmembrane protein and is known as a membrane endopeptidase. CD10 is expressed on predifferentiated lymphocyte progenitor, epithelial, stromal, and tumor cells. Antibodies against CD10 are used for the diagnosis of follicular lymphoma. Anti-human CD10 monoclonal antibody (clone MME/1870) can be used for Western blotting and immunohistochemical analyses. This study examined the critical epitope of MME/1870 using enzyme-linked immunosorbent assay (ELISA) with synthesized peptides. First, we performed ELISA with deletion mutants, and MME/1870 reacted to the 501–520 amino acid sequence of CD10. Next, we analyzed the reaction to 20 point mutants, and MME/1870 did not recognize the alanine-substituted peptides of Y507A, I511A, I512A, and L515A. These results indicate that the binding epitope of MME/1870 includes Tyr507, Ile511, Ile512, and Leu515 of CD10.

Original languageEnglish
Pages (from-to)15-19
Number of pages5
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Issue number1
Publication statusPublished - 2022 Feb 1


  • CD10
  • Clone MME/1870
  • Enzyme-linked immunosorbent assay
  • Epitope mapping
  • Monoclonal antibody

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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