TY - JOUR
T1 - Epitope Mapping of Antihuman Diacylglycerol Kinase Gamma Monoclonal Antibody DgMab-6
AU - Sano, Masato
AU - Kaneko, Mika K.
AU - Kato, Yukinari
N1 - Publisher Copyright:
© 2019, Mary Ann Liebert, Inc., publishers 2019.
PY - 2019/6
Y1 - 2019/6
N2 - Diacylglycerol kinase (DGK) converts diacylglycerol (DG) into phosphatidic acid (PA). Both DG and PA serve as lipidic second messengers; therefore, DGK plays a critical role in regulating the balance of two signaling pathways mediated by DG and PA in cellular functions. DGK gamma (DGKγ), 1 of the 10 mammalian DGK isozymes, has been reported to be involved in membrane traffic, mast cell function, and leukemic cell differentiation. We previously developed a novel anti-DGKγmonoclonal antibody (mAb), DgMab-6, which is very useful in immunocytochemistry of human cultured cells. In this study, we characterized the binding epitope of DgMab-6 using Western blotting, and Glu12 is important for facilitating the DgMab-6 binding to the DGKγprotein. These results might lead to further development for sensitive and specific mAbs against DGKγ.
AB - Diacylglycerol kinase (DGK) converts diacylglycerol (DG) into phosphatidic acid (PA). Both DG and PA serve as lipidic second messengers; therefore, DGK plays a critical role in regulating the balance of two signaling pathways mediated by DG and PA in cellular functions. DGK gamma (DGKγ), 1 of the 10 mammalian DGK isozymes, has been reported to be involved in membrane traffic, mast cell function, and leukemic cell differentiation. We previously developed a novel anti-DGKγmonoclonal antibody (mAb), DgMab-6, which is very useful in immunocytochemistry of human cultured cells. In this study, we characterized the binding epitope of DgMab-6 using Western blotting, and Glu12 is important for facilitating the DgMab-6 binding to the DGKγprotein. These results might lead to further development for sensitive and specific mAbs against DGKγ.
KW - DGKγ
KW - DgMab-6
KW - diacylglycerol kinase
KW - monoclonal antibody
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U2 - 10.1089/mab.2019.0011
DO - 10.1089/mab.2019.0011
M3 - Article
C2 - 31149888
AN - SCOPUS:85067300224
SN - 2167-9436
VL - 38
SP - 120
EP - 123
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
IS - 3
ER -