TY - JOUR
T1 - Epitope Mapping of Monoclonal Antibody PMab-48 Against Dog Podoplanin
AU - Yamada, Shinji
AU - Kaneko, Mika K.
AU - Itai, Shunsuke
AU - Chang, Yao Wen
AU - Nakamura, Takuro
AU - Yanaka, Miyuki
AU - Ogasawara, Satoshi
AU - Murata, Takeshi
AU - Uchida, Hiroaki
AU - Tahara, Hideaki
AU - Harada, Hiroyuki
AU - Kato, Yukinari
N1 - Funding Information:
The authors thank Noriko Saidoh, Saori Handa, and Yoshimi Nakamura for their excellent technical assistance. This research was supported, in part, by AMED under Grant Numbers: JP17am0301010 (Y.K.), JP17am0101078 (Y.K.), and JP17ae0101028 (Y.K.) and by JSPS KAKENHI Grant Number 17K07299 (M.K.K.) and Grant Number 16K10748 (Y.K.). This work was performed, in part, under the Co-operative Research Program of the Institute for Protein Research, Osaka University, CR-17-05, and by the Grant for Joint Research Project of the Institute of Medical Science, the University of Tokyo.
Publisher Copyright:
© Copyright 2018, Mary Ann Liebert, Inc. 2018.
PY - 2018/6
Y1 - 2018/6
N2 - Podoplanin (PDPN), a type I transmembrane sialoglycoprotein, is expressed on normal renal podocytes, pulmonary type I alveolar cells, and lymphatic endothelial cells. Increased expression of PDPN in cancers is associated with poor prognosis and hematogenous metastasis through interactions with C-type lectin-like receptor 2 (CLEC-2) on platelets. We previously reported a novel PMab-48 antibody, which is an anti-dog PDPN (dPDPN) monoclonal antibody (mAb) recognizing PDPN expressed in lymphatic endothelial cells. However, the binding epitope of PMab-48 is yet to be clarified. In this study, an enzyme-linked immunosorbent assay and flow cytometry were used to investigate epitopes of PMab-48. The results revealed that the critical epitope of PMab-48 comprises Asp29, Asp30, Ile31, Ile32, and Pro33 of dPDPN.
AB - Podoplanin (PDPN), a type I transmembrane sialoglycoprotein, is expressed on normal renal podocytes, pulmonary type I alveolar cells, and lymphatic endothelial cells. Increased expression of PDPN in cancers is associated with poor prognosis and hematogenous metastasis through interactions with C-type lectin-like receptor 2 (CLEC-2) on platelets. We previously reported a novel PMab-48 antibody, which is an anti-dog PDPN (dPDPN) monoclonal antibody (mAb) recognizing PDPN expressed in lymphatic endothelial cells. However, the binding epitope of PMab-48 is yet to be clarified. In this study, an enzyme-linked immunosorbent assay and flow cytometry were used to investigate epitopes of PMab-48. The results revealed that the critical epitope of PMab-48 comprises Asp29, Asp30, Ile31, Ile32, and Pro33 of dPDPN.
KW - dog podoplanin
KW - dPDPN
KW - epitope mapping
KW - monoclonal antibody
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U2 - 10.1089/mab.2018.0006
DO - 10.1089/mab.2018.0006
M3 - Article
C2 - 29608407
AN - SCOPUS:85049077017
SN - 2167-9436
VL - 37
SP - 162
EP - 165
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
IS - 3
ER -
