TY - JOUR
T1 - Epitope Mapping of the Antihorse Podoplanin Monoclonal Antibody PMab-202
AU - Kaneko, Mika K.
AU - Furusawa, Yoshikazu
AU - Sano, Masato
AU - Itai, Shunsuke
AU - Takei, Junko
AU - Harada, Hiroyuki
AU - Fukui, Masato
AU - Yamada, Shinji
AU - Kato, Yukinari
N1 - Funding Information:
We thank Takuro Nakamura, Miyuki Yanaka, Saori Handa, Kayo Hisamatsu, and Yoshimi Nakamura for excellent technical assistance. FHK-Tcl3.1 was kindly provided by Ken Maeda (Yamaguchi University). This research was supported, in part, by AMED under Grant Nos.: JP18am0101078 (Y.K.), JP18am0301010 (Y.K.), and JP18ae0101028 (Y.K.), and by JSPS KAKENHI Grant No. 17K07299 (M.K.K.) and Grant No. 16K10748 (Y.K.).
Funding Information:
Y.K. received research funding from ZENOAQ Resource Co., Ltd. The other authors have no conflict of interest.
Publisher Copyright:
© 2019, Mary Ann Liebert, Inc.
PY - 2019/4/1
Y1 - 2019/4/1
N2 - Horse podoplanin (horPDPN), a type I transmembrane sialoglycoprotein, is expressed on the podocytes of the kidneys, alveolar type I cells of the lungs, and lymphatic endothelial cells. PDPN is a platelet aggregation-inducing factor, and it primarily possesses three platelet aggregation-stimulating (PLAG) domains, that is, PLAG1, PLAG2, and PLAG3, at the N-terminus and several PLAG-like domains. In a previous study, we reported on a mouse anti-horPDPN monoclonal antibody (mAb) clone, PMab-202. Although the effectiveness of PMab-202 in flow cytometry and Western blotting is known, its exact binding epitope remains unknown to date. In this study, enzyme-linked immunosorbent assay and flow cytometry were used to identify the epitope of PMab-202. We found that the critical epitopes of PMab-202 include Lys64, Thr66, and Phe70 of horPDPN. We believe that our findings can be applied in the production of more functional anti-horPDPN mAbs.
AB - Horse podoplanin (horPDPN), a type I transmembrane sialoglycoprotein, is expressed on the podocytes of the kidneys, alveolar type I cells of the lungs, and lymphatic endothelial cells. PDPN is a platelet aggregation-inducing factor, and it primarily possesses three platelet aggregation-stimulating (PLAG) domains, that is, PLAG1, PLAG2, and PLAG3, at the N-terminus and several PLAG-like domains. In a previous study, we reported on a mouse anti-horPDPN monoclonal antibody (mAb) clone, PMab-202. Although the effectiveness of PMab-202 in flow cytometry and Western blotting is known, its exact binding epitope remains unknown to date. In this study, enzyme-linked immunosorbent assay and flow cytometry were used to identify the epitope of PMab-202. We found that the critical epitopes of PMab-202 include Lys64, Thr66, and Phe70 of horPDPN. We believe that our findings can be applied in the production of more functional anti-horPDPN mAbs.
KW - epitope mapping
KW - PDPN
KW - PMab-202
KW - podoplanin
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U2 - 10.1089/mab.2019.0001
DO - 10.1089/mab.2019.0001
M3 - Article
C2 - 30939066
AN - SCOPUS:85065111899
SN - 2167-9436
VL - 38
SP - 79
EP - 84
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
IS - 2
ER -