EPR spin-trapping study of nitric oxide formation during bilateral carotid occlusion in the rat

Shinya Sato; Teiji Tominaga; Tomoko Ohnishi; S. Tsuyoshi Ohnishi

doi:10.1016/0925-4439(93)90111-D

EPR spin-trapping study of nitric oxide formation during bilateral carotid occlusion in the rat

Shinya Sato, Teiji Tominaga, Tomoko Ohnishi, S. Tsuyoshi Ohnishi

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Abstract

The formation of nitric oxide (NO) radicals was demonstrated by electron paramagnetic resonance spectroscopy in the rat during varying degrees of brain ischemia. Diethyldithiocarbamate and Fe-citrate were used as in vivo spin-trapping reagents. The signal of NO spin adducts increased in accordance with the degree of ischemic insults. The formation of NO radicals was inhibited by N^G-nitro]l-arginine methyl ester.

Original language	English
Pages (from-to)	195-197
Number of pages	3
Journal	Biochimica et Biophysica Acta - Molecular Basis of Disease
Volume	1181
Issue number	2
DOIs	https://doi.org/10.1016/0925-4439(93)90111-D
Publication status	Published - 1993 Apr 30

Keywords

Brian ischemia
Common carotid occlusion
EPR
Nitric oxide (NO)
Spin-trapping

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10.1016/0925-4439(93)90111-D

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title = "EPR spin-trapping study of nitric oxide formation during bilateral carotid occlusion in the rat",

abstract = "The formation of nitric oxide (NO) radicals was demonstrated by electron paramagnetic resonance spectroscopy in the rat during varying degrees of brain ischemia. Diethyldithiocarbamate and Fe-citrate were used as in vivo spin-trapping reagents. The signal of NO spin adducts increased in accordance with the degree of ischemic insults. The formation of NO radicals was inhibited by NG-nitro]l-arginine methyl ester.",

keywords = "Brian ischemia, Common carotid occlusion, EPR, Nitric oxide (NO), Spin-trapping",

author = "Shinya Sato and Teiji Tominaga and Tomoko Ohnishi and Ohnishi, {S. Tsuyoshi}",

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T1 - EPR spin-trapping study of nitric oxide formation during bilateral carotid occlusion in the rat

AU - Sato, Shinya

AU - Tominaga, Teiji

AU - Ohnishi, Tomoko

AU - Ohnishi, S. Tsuyoshi

N1 - Funding Information: tute of Brain Diseases,T ohoku UniversityS chool of Medicine for his encouragemenTth. e work was supported by NIH Grants NS30196 to S.T.O. and GM30736t o T.O.

PY - 1993/4/30

Y1 - 1993/4/30

N2 - The formation of nitric oxide (NO) radicals was demonstrated by electron paramagnetic resonance spectroscopy in the rat during varying degrees of brain ischemia. Diethyldithiocarbamate and Fe-citrate were used as in vivo spin-trapping reagents. The signal of NO spin adducts increased in accordance with the degree of ischemic insults. The formation of NO radicals was inhibited by NG-nitro]l-arginine methyl ester.

AB - The formation of nitric oxide (NO) radicals was demonstrated by electron paramagnetic resonance spectroscopy in the rat during varying degrees of brain ischemia. Diethyldithiocarbamate and Fe-citrate were used as in vivo spin-trapping reagents. The signal of NO spin adducts increased in accordance with the degree of ischemic insults. The formation of NO radicals was inhibited by NG-nitro]l-arginine methyl ester.

KW - Brian ischemia

KW - Common carotid occlusion

KW - EPR

KW - Nitric oxide (NO)

KW - Spin-trapping

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EPR spin-trapping study of nitric oxide formation during bilateral carotid occlusion in the rat

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Keywords

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