TY - JOUR
T1 - Ergosterol-Induced Ordered Phase in Ternary Lipid Mixture Systems of Unsaturated and Saturated Phospholipid Membranes
AU - Bui, Tham Thi
AU - Suga, Keishi
AU - Umakoshi, Hiroshi
N1 - Funding Information:
This work was primarily supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant-in-Aids for Scientific Research (A) (26249116), Young Scientists (B) (16K18279), Challenging Exploratory Research (T15K142040), and Early-Career Scientists (19K15338). T.T.B. is grateful for support from a Japanese Government (Monbukagakusho: MEXT) scholarship while completing this study. K.S. expresses his gratitude to the COSMETOLOGY Research Foundation and Multidisciplinary Research Laboratory System of Osaka University.
Funding Information:
This work was primarily supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant-in-Aids for Scientific Research (A) (26249116), Young Scientists (B) (16K18279), Challenging Exploratory Research (T15K142040), and Early-Career Scientists (19K15338). T.T.B. is grateful for support from a Japanese Government (Monbukagakusho: MEXT) scholarship while completing this study. K.S. expresses his gratitude to the COSMETOLOGY Research Foundation and Multidisciplinary Research Laboratory System of Osaka University.
Publisher Copyright:
© 2019 American Chemical Society.
PY - 2019/7/1
Y1 - 2019/7/1
N2 - The sterol ergosterol (Erg) is ubiquitous in the membranes of lower eukaryotes such as fungi. To investigate the interactions between Erg and phosphocholine (PC) molecules, we studied ternary lipid mixture systems composed of unsaturated 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), saturated 1,2-dipalmitoyl-sn-glycero-3-phophocholine (DPPC), and Erg. Bilayer membrane fluidity and polarity were systematically analyzed using fluorescent probes. The presence of ≥30 mol % of Erg exhibited a significant ordering effect and stabilized membrane properties when temperatures increased, suggesting that Erg has a similar function to cholesterol (Chol) in comparable lipid systems that form a liquid-ordered phase. Erg was also observed to have a significant condensing effect at the monolayer level in saturated PC-enriched systems. The phase behavior of Erg in bilayer systems was compared with that of Chol, with the data suggesting that Erg behaves in a similar manner to Chol in membranes enriched with saturated lipids.
AB - The sterol ergosterol (Erg) is ubiquitous in the membranes of lower eukaryotes such as fungi. To investigate the interactions between Erg and phosphocholine (PC) molecules, we studied ternary lipid mixture systems composed of unsaturated 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), saturated 1,2-dipalmitoyl-sn-glycero-3-phophocholine (DPPC), and Erg. Bilayer membrane fluidity and polarity were systematically analyzed using fluorescent probes. The presence of ≥30 mol % of Erg exhibited a significant ordering effect and stabilized membrane properties when temperatures increased, suggesting that Erg has a similar function to cholesterol (Chol) in comparable lipid systems that form a liquid-ordered phase. Erg was also observed to have a significant condensing effect at the monolayer level in saturated PC-enriched systems. The phase behavior of Erg in bilayer systems was compared with that of Chol, with the data suggesting that Erg behaves in a similar manner to Chol in membranes enriched with saturated lipids.
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U2 - 10.1021/acs.jpcb.9b03413
DO - 10.1021/acs.jpcb.9b03413
M3 - Article
C2 - 31260623
AN - SCOPUS:85070605975
SN - 1520-6106
VL - 123
SP - 6161
EP - 6168
JO - Journal of Physical Chemistry B
JF - Journal of Physical Chemistry B
IS - 29
ER -