TY - JOUR
T1 - Establishment and characterization of a novel ovarian serous adenocarcinoma cell line, TU-OS-4, that overexpresses EGFR and HER2
AU - Itamochi, Hiroaki
AU - Kato, Misaki
AU - Nishimura, Mayumi
AU - Oishi, Tetsuro
AU - Shimada, Muneaki
AU - Sato, Shinya
AU - Naniwa, Jun
AU - Sato, Seiya
AU - Nonaka, Michiko
AU - Kudoh, Akiko
AU - Terakawa, Naoki
AU - Kigawa, Junzo
AU - Harada, Tasuku
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (17244120 to H. Itamochi).
PY - 2012/12
Y1 - 2012/12
N2 - A new line of human ovarian serous adenocarcinoma cells, TU-OS-4, was established and characterized. The cells showed a short, spindle-shaped morphology and grew in monolayers without contact inhibition while forming an arrangement resembling a jigsaw puzzle. Chromosome numbers ranged from 55 to 73. The proliferation rate was lower than other serous adenocarcinoma cell lines tested (KF, SHIN-3, and SK-OV-3), and the doubling time was 53. 3 h. Western blot analysis showed that TU-OS-4 cells overexpressed epidermal growth factor receptor, human epidermal growth factor receptor (HER) 2, and phosphorylated HER2 protein. The IC50 values to cisplatin, paclitaxel, and lapatinib were 25. 8 μM, 686 nM, and 183 nM, respectively. Heterotransplantation in nude mice reflected the original tumor of the cells. These results suggested that this cell line would be useful to study chemoresistant mechanisms and contribute to establishing novel treatment strategies for patients with ovarian cancer.
AB - A new line of human ovarian serous adenocarcinoma cells, TU-OS-4, was established and characterized. The cells showed a short, spindle-shaped morphology and grew in monolayers without contact inhibition while forming an arrangement resembling a jigsaw puzzle. Chromosome numbers ranged from 55 to 73. The proliferation rate was lower than other serous adenocarcinoma cell lines tested (KF, SHIN-3, and SK-OV-3), and the doubling time was 53. 3 h. Western blot analysis showed that TU-OS-4 cells overexpressed epidermal growth factor receptor, human epidermal growth factor receptor (HER) 2, and phosphorylated HER2 protein. The IC50 values to cisplatin, paclitaxel, and lapatinib were 25. 8 μM, 686 nM, and 183 nM, respectively. Heterotransplantation in nude mice reflected the original tumor of the cells. These results suggested that this cell line would be useful to study chemoresistant mechanisms and contribute to establishing novel treatment strategies for patients with ovarian cancer.
KW - Chemosensitivity
KW - Epidermal growth factor receptor
KW - Establishment
KW - Molecular targeted therapy
KW - Ovarian serous adenocarcinoma
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U2 - 10.1007/s13577-012-0048-1
DO - 10.1007/s13577-012-0048-1
M3 - Article
C2 - 23274876
AN - SCOPUS:84872650322
SN - 0914-7470
VL - 25
SP - 111
EP - 115
JO - Human Cell
JF - Human Cell
IS - 4
ER -
