TY - JOUR
T1 - Establishment and Epitope Mapping of Anti-Diacylglycerol Kinase α Monoclonal Antibody DaMab-8 for Immunohistochemical Analyses
AU - Sano, Masato
AU - Kaneko, Mika K.
AU - Suzuki, Hiroyoshi
AU - Kato, Yukinari
N1 - Funding Information:
Y.K. received research funding from Ono Pharmaceutical Co., Ltd. The other authors have no conflict of interest.
Funding Information:
We thank Shunsuke Itai, Miyuki Yanaka, Kayo Hisamatsu, Saori Handa, Yoshimi Nakamura, and Maki Takahashi for their excellent technical assistance, and Shinji Yamada for his helpful discussion. This research was supported, in part, by AMED under Grant Numbers: JP18am0101te078 (Y.K.), JP18am0301010 (Y.K.), and JP18ae0101028 (Y.K.), and by JSPS KAKENHI Grant Numbers 17K07299 (M.K.K.) and 16K10748 (Y.K.).
Publisher Copyright:
© Masato Sano et al. 2019; Published by Mary Ann Liebert, Inc. 2019.
PY - 2020/2
Y1 - 2020/2
N2 - Diacylglycerol kinase (DGK) converts diacylglycerol (DG) into phosphatidic acid (PA). DGKα, 1 of the 10 DGK isozymes, is involved in T cell function. In the present study, we describe a specific monoclonal antibody DaMab-8 (mouse IgG1, kappa) against DGKα, which is extremely useful for performing immunohistochemical analysis for T cells in oropharyngeal squamous cell carcinomas. Furthermore, we characterized the binding epitope of DaMab-8 using Western blotting and found that the sites Asn610, Leu611, Trp612, Gly613, Asp614, His619, Tyr623, and Gly624 of DGKα are important for facilitating the DaMab-8 binding to the DGKα protein. Thus, DaMab-8 could be advantageous for immunohistochemical analyses toward clarifying the distribution of DGKα-expressing T cells in every pathophysiological tissue.
AB - Diacylglycerol kinase (DGK) converts diacylglycerol (DG) into phosphatidic acid (PA). DGKα, 1 of the 10 DGK isozymes, is involved in T cell function. In the present study, we describe a specific monoclonal antibody DaMab-8 (mouse IgG1, kappa) against DGKα, which is extremely useful for performing immunohistochemical analysis for T cells in oropharyngeal squamous cell carcinomas. Furthermore, we characterized the binding epitope of DaMab-8 using Western blotting and found that the sites Asn610, Leu611, Trp612, Gly613, Asp614, His619, Tyr623, and Gly624 of DGKα are important for facilitating the DaMab-8 binding to the DGKα protein. Thus, DaMab-8 could be advantageous for immunohistochemical analyses toward clarifying the distribution of DGKα-expressing T cells in every pathophysiological tissue.
KW - DGKα
KW - diacylglycerol kinase
KW - epitope mapping
KW - monoclonal antibody
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U2 - 10.1089/mab.2019.0004
DO - 10.1089/mab.2019.0004
M3 - Article
C2 - 31099705
AN - SCOPUS:85079820113
SN - 2167-9436
VL - 39
SP - 1
EP - 5
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
IS - 1
ER -