TY - JOUR
T1 - Establishment and mutation analysis of a novel malignant peritoneal mesothelioma cell line, TU-MM-1, using whole genome sequencing
AU - Oumi, Nao
AU - Itamochi, Hiroaki
AU - Komatsu, Hiroaki
AU - Oishi, Tetsuro
AU - Shimada, Muneaki
AU - Sato, Shinya
AU - Chikumi, Jun
AU - Sato, Seiya
AU - Nonaka, Michiko
AU - Kudoh, Akiko
AU - Harada, Tasuku
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (17244120 to H. Itamochi).
Publisher Copyright:
© 2015, Japan Human Cell Society and Springer Japan.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - A new cell line of human malignant peritoneal mesothelioma (MPM), TU-MM-1, was established and characterized. The cells showed polygonal morphology, grew in monolayers without contact inhibition and were arranged like a jigsaw puzzle. The chromosome numbers ranged from 41 to 44. A low rate of proliferation was observed and the doubling time was 67.9 h. Genomic DNA sequencing revealed that TU-MM-1 cells harbored missense mutations in APC, LATS2, BRCA1/2, and TP53, and mutation of a splice donor site in BAP1 and loss of CDKN2A gene. We observed the absence of BAP1 and p16INK4a proteins, underexpression of LATS2 protein, and overexpression of p53 protein in TU-MM-1 cells in western blot analysis. Heterotransplantation to nude mice produced tumors that had the characteristics of the original tumor. This cell line may be useful for studying biological properties and contribute to novel treatment strategies.
AB - A new cell line of human malignant peritoneal mesothelioma (MPM), TU-MM-1, was established and characterized. The cells showed polygonal morphology, grew in monolayers without contact inhibition and were arranged like a jigsaw puzzle. The chromosome numbers ranged from 41 to 44. A low rate of proliferation was observed and the doubling time was 67.9 h. Genomic DNA sequencing revealed that TU-MM-1 cells harbored missense mutations in APC, LATS2, BRCA1/2, and TP53, and mutation of a splice donor site in BAP1 and loss of CDKN2A gene. We observed the absence of BAP1 and p16INK4a proteins, underexpression of LATS2 protein, and overexpression of p53 protein in TU-MM-1 cells in western blot analysis. Heterotransplantation to nude mice produced tumors that had the characteristics of the original tumor. This cell line may be useful for studying biological properties and contribute to novel treatment strategies.
KW - BRCA1/2
KW - Establishment
KW - Malignant peritoneal mesothelioma
KW - TP53
KW - Whole genome sequence
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U2 - 10.1007/s13577-015-0120-8
DO - 10.1007/s13577-015-0120-8
M3 - Article
C2 - 26070481
AN - SCOPUS:84953638777
SN - 0914-7470
VL - 29
SP - 46
EP - 51
JO - Human Cell
JF - Human Cell
IS - 1
ER -
