TY - JOUR
T1 - Establishment of a Monoclonal Antibody That Recognizes Cysteine-Rich Domain 1 of Human CD271
AU - Morita, Shinkichi
AU - Mochizuki, Mai
AU - Shibuya-Takahashi, Rie
AU - Nakamura-Shima, Mao
AU - Yamazaki, Tomoko
AU - Imai, Takayuki
AU - Asada, Yukinori
AU - Matsuura, Kazuto
AU - Kawamura, Sadafumi
AU - Yamaguchi, Kazunori
AU - Yasuda, Jun
AU - Sugamura, Kazuo
AU - Katori, Yukio
AU - Satoh, Kennichi
AU - Tamai, Keiichi
N1 - Funding Information:
This research was supported, in part, by Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Nos. JP: 19K08430, 17K10716, 17K11166, 16K07132, 18K16432, and 19K09903 and Practical Research for Innovative Cancer Control from Japan Agency for Medical Research and Development (AMED) by Grant No. JP17ck0106197, The Cooperative Research Project Program of Joint Usage/Research Center at The Institute of Development, Aging and Cancer, Tohoku University, and Takeda Medical Foundation.
Publisher Copyright:
© Copyright 2020, Mary Ann Liebert, Inc., publishers 2020.
PY - 2020/2
Y1 - 2020/2
N2 - CD271 is a common receptor for all neurotrophins that is localized to neurons, endothelial cells, and the basal layer of the epithelium in normal tissue. Recently, we and others reported that CD271 plays essential roles in the development of squamous cell carcinoma, especially in tumor-initiating cells. Since little is known about how CD271 regulates cancer cell initiation and proliferation, antibodies that recognize different domains of CD271 are needed to enable investigation. Therefore, this study aimed to develop an antihuman CD271 antibody by immunizing mice with a CD271 antigen produced by a baculovirus. The antibody was named hCD271mAb#13, and it recognized cysteine-rich domain 1 with a higher affinity than the commercially available antibody ME20.4. We determined that hCD271mAb#13 is suitable for flow cytometry, Western blotting, immunocytochemistry, and immunohistochemistry of formalin-fixed paraffin-embedded tissue. Use of hCD271mAb#13 for CD271 labeling could enable detailed analyses of cancer cell regulation and other biological processes.
AB - CD271 is a common receptor for all neurotrophins that is localized to neurons, endothelial cells, and the basal layer of the epithelium in normal tissue. Recently, we and others reported that CD271 plays essential roles in the development of squamous cell carcinoma, especially in tumor-initiating cells. Since little is known about how CD271 regulates cancer cell initiation and proliferation, antibodies that recognize different domains of CD271 are needed to enable investigation. Therefore, this study aimed to develop an antihuman CD271 antibody by immunizing mice with a CD271 antigen produced by a baculovirus. The antibody was named hCD271mAb#13, and it recognized cysteine-rich domain 1 with a higher affinity than the commercially available antibody ME20.4. We determined that hCD271mAb#13 is suitable for flow cytometry, Western blotting, immunocytochemistry, and immunohistochemistry of formalin-fixed paraffin-embedded tissue. Use of hCD271mAb#13 for CD271 labeling could enable detailed analyses of cancer cell regulation and other biological processes.
KW - CD271
KW - CRD1
KW - immunohistochemistry
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U2 - 10.1089/mab.2019.0040
DO - 10.1089/mab.2019.0040
M3 - Article
C2 - 32069133
AN - SCOPUS:85079696944
SN - 2167-9436
VL - 39
SP - 6
EP - 11
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
IS - 1
ER -
