TY - JOUR
T1 - Establishment of immortalized primary cell from the critically endangered Bonin flying fox (Pteropus pselaphon)
AU - Tani, Tetsuya
AU - Eitsuka,
AU - Katayama,
AU - Nagamine,
AU - Nakaya, Yumiko
AU - Suzuki, Hajime
AU - Kiyono, Tohru
AU - Nakagawa, Kiyotaka
AU - Inoue-Murayama, Miho
AU - Onuma, Manabu
AU - Fukuda, Tomokazu
N1 - Funding Information:
Funding:ThisworkwassupportedbyJapan Societyforthepromotionofscience(JSPS) KAKENHI(Grantnumber,17H05810).Thisproject issupportedbyGrants-in-AidforScientific ResearchfromtheMinistryofEducation,Culture, Sports,Science,andTechnology,andresearch grantfromWaksmanFoundationofJapaninc.The fundershadnoroleinstudydesign,datacollection
Funding Information:
This work was supported by Japan Society for the promotion of science (JSPS) KAKENHI (Grant number, 17H05810). This project is supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology, and research grant from Waksman Foundation of Japan inc. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank Dr. Hiroyuki Miyoshi (RIKEN, BioResource Center, Tsukuba, Japan) for providing the lentiviral plasmids and packaging system.
Publisher Copyright:
© 2019 Tani et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2019/8/1
Y1 - 2019/8/1
N2 - The Bonin flying fox (Pteropus pselaphon) is one of the most critically endangered species of animals. The number of this species is estimated to be around 150; being classified at the top rank in the list by International Union of Animal Conservation. Our group previously showed that expression of CDK4, CYCLIN D1, and telomerase reverse transcriptase (TERT) efficiently induce immortalization of human, bovine, swine, monkey, and buffalo-derived cells. In this manuscript, we successfully established the primary cells from Bonin flying fox. We introduced CDK4, CYCLIN D1, and TERT into the primary cells. The established cells showed efficient expression of introduced genes at the protein level. Furthermore, the established cells were free from senescence, indicating it reached to immortalization. Moreover, we showed that interspecies somatic cell nuclear transfer of Bonin flying fox derived cell into bovine embryo allowed the development of the embryo to 8 cell stages. Our established cell has the potential to contribute to species conservation.
AB - The Bonin flying fox (Pteropus pselaphon) is one of the most critically endangered species of animals. The number of this species is estimated to be around 150; being classified at the top rank in the list by International Union of Animal Conservation. Our group previously showed that expression of CDK4, CYCLIN D1, and telomerase reverse transcriptase (TERT) efficiently induce immortalization of human, bovine, swine, monkey, and buffalo-derived cells. In this manuscript, we successfully established the primary cells from Bonin flying fox. We introduced CDK4, CYCLIN D1, and TERT into the primary cells. The established cells showed efficient expression of introduced genes at the protein level. Furthermore, the established cells were free from senescence, indicating it reached to immortalization. Moreover, we showed that interspecies somatic cell nuclear transfer of Bonin flying fox derived cell into bovine embryo allowed the development of the embryo to 8 cell stages. Our established cell has the potential to contribute to species conservation.
UR - http://www.scopus.com/inward/record.url?scp=85071290682&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85071290682&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0221364
DO - 10.1371/journal.pone.0221364
M3 - Article
C2 - 31449544
AN - SCOPUS:85071290682
SN - 1932-6203
VL - 14
JO - PLoS One
JF - PLoS One
IS - 8
M1 - e0221364
ER -