TY - JOUR
T1 - Establishment of Novel Monoclonal Antibody PMab-32 Against Rabbit Podoplanin
AU - Honma, Ryusuke
AU - Fujii, Yuki
AU - Ogasawara, Satoshi
AU - Oki, Hiroharu
AU - Liu, Xing
AU - Nakamura, Takuro
AU - Kaneko, Mika K.
AU - Takagi, Michiaki
AU - Kato, Yukinari
N1 - Publisher Copyright:
© Copyright 2016, Mary Ann Liebert, Inc.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - Podoplanin (PDPN) is a type I transmembrane O-glycoprotein, which is known as a specific lymphatic marker. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) on platelet. PDPN is also expressed in several normal tissues, including podocytes and type I alveolar cells. Although many monoclonal antibodies (MAbs) against human PDPN (hPDPN), mouse PDPN (mPDPN), and rat PDPN (rPDPN) have been established, useful antibodies against rabbit PDPN (rabPDPN) have not been developed. In this study, we immunized mice with the recombinant proteins of rabPDPN, and developed a novel anti-rabPDPN MAb, named PMab-32. PMab-32 could detect endogenous and exogenous rabPDPN in flow cytometry and Western blot analysis. The KD of PMab-32 was determined to be 6.2 × 10-8 M by flow cytometry. Immunohistochemical analysis showed that PMab-32 is useful for detecting podocytes, type I alveolar cells, and lymphatic endothelial cells in normal rabbit tissues. PMab-32 is expected to be useful for various rabbit experiments.
AB - Podoplanin (PDPN) is a type I transmembrane O-glycoprotein, which is known as a specific lymphatic marker. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) on platelet. PDPN is also expressed in several normal tissues, including podocytes and type I alveolar cells. Although many monoclonal antibodies (MAbs) against human PDPN (hPDPN), mouse PDPN (mPDPN), and rat PDPN (rPDPN) have been established, useful antibodies against rabbit PDPN (rabPDPN) have not been developed. In this study, we immunized mice with the recombinant proteins of rabPDPN, and developed a novel anti-rabPDPN MAb, named PMab-32. PMab-32 could detect endogenous and exogenous rabPDPN in flow cytometry and Western blot analysis. The KD of PMab-32 was determined to be 6.2 × 10-8 M by flow cytometry. Immunohistochemical analysis showed that PMab-32 is useful for detecting podocytes, type I alveolar cells, and lymphatic endothelial cells in normal rabbit tissues. PMab-32 is expected to be useful for various rabbit experiments.
UR - http://www.scopus.com/inward/record.url?scp=84958818058&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84958818058&partnerID=8YFLogxK
U2 - 10.1089/mab.2015.0066
DO - 10.1089/mab.2015.0066
M3 - Article
C2 - 26788987
AN - SCOPUS:84958818058
SN - 2167-9436
VL - 35
SP - 41
EP - 47
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
IS - 1
ER -