TY - JOUR
T1 - Estimation of oxygen metabolism in a rat model of permanent ischemia using positron emission tomography with injectable15O-O2
AU - Temma, Takashi
AU - Magata, Yasuhiro
AU - Kuge, Yuji
AU - Shimonaka, Sayaka
AU - Sano, Kohei
AU - Katada, Yumiko
AU - Kawashima, Hidekazu
AU - Mukai, Takahiro
AU - Watabe, Hiroshi
AU - Iida, Hidehiro
AU - Saji, Hideo
PY - 2006/12/8
Y1 - 2006/12/8
N2 - The threshold of cerebral blood flow (CBF) into infarction in rats has been indicated to be similar to that in patients. However, CBF does not reflect metabolic function, and so estimations of oxygen metabolism have been required. Here, we estimated changes in oxygen metabolism after occluding the right middle cerebral artery (MCA) in rats using an injectable 15O-O2 we developed. A decrease in CBF (left: 0.67±0.22 mL/min/g, right: 0.44±0.17 mL/min/g, P<0.05) and compensatory increase in the oxygen extraction fraction (OEF) (left: 0.42±0.13, right: 0.50±0.19, P<0.05) were observed at 1-h after occlusion. In contrast, a marked decrease in CBF and the cerebral metabolic rate for oxygen and a collapse of the compensatory OEF mechanism were found at 24 h after occlusion. Injectable 15O-O2 could be used to reliably estimate oxygen metabolism in an infarction rat model with positron emission tomography.
AB - The threshold of cerebral blood flow (CBF) into infarction in rats has been indicated to be similar to that in patients. However, CBF does not reflect metabolic function, and so estimations of oxygen metabolism have been required. Here, we estimated changes in oxygen metabolism after occluding the right middle cerebral artery (MCA) in rats using an injectable 15O-O2 we developed. A decrease in CBF (left: 0.67±0.22 mL/min/g, right: 0.44±0.17 mL/min/g, P<0.05) and compensatory increase in the oxygen extraction fraction (OEF) (left: 0.42±0.13, right: 0.50±0.19, P<0.05) were observed at 1-h after occlusion. In contrast, a marked decrease in CBF and the cerebral metabolic rate for oxygen and a collapse of the compensatory OEF mechanism were found at 24 h after occlusion. Injectable 15O-O2 could be used to reliably estimate oxygen metabolism in an infarction rat model with positron emission tomography.
KW - OEF
KW - Oxygen metabolism
KW - Permanent ischemia
KW - Positron emission tomography
KW - Rat
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UR - http://www.scopus.com/inward/citedby.url?scp=33751346575&partnerID=8YFLogxK
U2 - 10.1038/sj.jcbfm.9600302
DO - 10.1038/sj.jcbfm.9600302
M3 - Article
C2 - 16552423
AN - SCOPUS:33751346575
SN - 0271-678X
VL - 26
SP - 1577
EP - 1583
JO - Journal of Cerebral Blood Flow and Metabolism
JF - Journal of Cerebral Blood Flow and Metabolism
IS - 12
ER -