TY - JOUR
T1 - Ethanol prefermentation of food waste in sequencing batch methane fermentation for improved buffering capacity and microbial community analysis
AU - Yu, Miao
AU - Wu, Chuanfu
AU - Wang, Qunhui
AU - Sun, Xiaohong
AU - Ren, Yuanyuan
AU - Li, Yu You
N1 - Funding Information:
This study was supported by the National Natural Science Foundation of China (Grant No. 51278063 ) and the National Key Technology R&D Program ( 2014BAC24B01 ).
Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2018/1
Y1 - 2018/1
N2 - This study investigates the effects of ethanol prefermentation (EP) on methane fermentation. Yeast was added to the substrate for EP in the sequencing batch methane fermentation of food waste. An Illumina MiSeq high-throughput sequencing system was used to analyze changes in the microbial community. Methane production in the EP group (254 mL/g VS) was higher than in the control group (35 mL/g VS) because EP not only increased the buffering capacity of the system, but also increased hydrolytic acidification. More carbon source was converted to ethanol in the EP group than in the control group, and neutral ethanol could be converted continuously to acetic acid, which promoted the growth of Methanobacterium and Methanosarcina. As a result, the relative abundance of methane-producing bacteria was significantly higher than that of the control group. Kinetic modeling indicated that the EP group had a higher hydrolysis efficiency and shorter lag phase.
AB - This study investigates the effects of ethanol prefermentation (EP) on methane fermentation. Yeast was added to the substrate for EP in the sequencing batch methane fermentation of food waste. An Illumina MiSeq high-throughput sequencing system was used to analyze changes in the microbial community. Methane production in the EP group (254 mL/g VS) was higher than in the control group (35 mL/g VS) because EP not only increased the buffering capacity of the system, but also increased hydrolytic acidification. More carbon source was converted to ethanol in the EP group than in the control group, and neutral ethanol could be converted continuously to acetic acid, which promoted the growth of Methanobacterium and Methanosarcina. As a result, the relative abundance of methane-producing bacteria was significantly higher than that of the control group. Kinetic modeling indicated that the EP group had a higher hydrolysis efficiency and shorter lag phase.
KW - Ethanol prefermentation
KW - Food waste
KW - High-throughput sequencing
KW - Methane fermentation
KW - Microbial community
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U2 - 10.1016/j.biortech.2017.07.013
DO - 10.1016/j.biortech.2017.07.013
M3 - Article
C2 - 28743613
AN - SCOPUS:85025102270
SN - 0960-8524
VL - 248
SP - 187
EP - 193
JO - Bioresource Technology
JF - Bioresource Technology
ER -
