TY - JOUR
T1 - Evaluation of antiherpetic compounds using a gastric cancer cell line
T2 - Pronounced activity of BVDU against herpes simplex virus replication
AU - Kodama, Eiichi
AU - Igarashi, Akira
AU - Mori, Shuichi
AU - Hashimoto, Kou Ichi
AU - Suzuki, Toshimitsu
AU - De Clercq, Erik
AU - Shigeta, Shiro
PY - 1996
Y1 - 1996
N2 - We developed a rapid and simple method for the screening of antiviral agents against herpes simplex virus (HSV) in a model of gastrointestinal herpetic infection in vitro. This method was based on inhibition of HSV- induced cytopathogenicity in gastric adenocarcinoma MKN-28 cells, as monitored by an MTT colorimetric assay. From the various compounds that were evaluated for their activity against HSV-1 and HSV-2, brivudine (BVDU) emerged as the most effective. When the 50% effective concentration (EC50) values of the antiherpes agents in MKN-28 cells were compared with those in human embryo lung MRC-5 cells, all compounds, except for BVDU, showed higher EC50 values in MKN-28 cells. For BVDU the EC50 values in MKN-28 cells were 0.8 (HSV-1) and 0.036 (HSV-2) times the EC50 values in MRC-5 cells. Thus BVDU was 27.5 times more active against HSV-2 in MKN-28 cells than in MRC-5 cells. The MKN-28 gastric cancer cells may be useful for the rapid screening of anti-HSV agents and, in particular, those that may he useful in therapy of gastrointestinal HSV infections in gastrointestinal herpetic infection.
AB - We developed a rapid and simple method for the screening of antiviral agents against herpes simplex virus (HSV) in a model of gastrointestinal herpetic infection in vitro. This method was based on inhibition of HSV- induced cytopathogenicity in gastric adenocarcinoma MKN-28 cells, as monitored by an MTT colorimetric assay. From the various compounds that were evaluated for their activity against HSV-1 and HSV-2, brivudine (BVDU) emerged as the most effective. When the 50% effective concentration (EC50) values of the antiherpes agents in MKN-28 cells were compared with those in human embryo lung MRC-5 cells, all compounds, except for BVDU, showed higher EC50 values in MKN-28 cells. For BVDU the EC50 values in MKN-28 cells were 0.8 (HSV-1) and 0.036 (HSV-2) times the EC50 values in MRC-5 cells. Thus BVDU was 27.5 times more active against HSV-2 in MKN-28 cells than in MRC-5 cells. The MKN-28 gastric cancer cells may be useful for the rapid screening of anti-HSV agents and, in particular, those that may he useful in therapy of gastrointestinal HSV infections in gastrointestinal herpetic infection.
KW - Antiviral screening
KW - BVDU
KW - Gastric cancer cell line
KW - Herpes simplex virus
KW - MTT method
UR - http://www.scopus.com/inward/record.url?scp=0029941889&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029941889&partnerID=8YFLogxK
U2 - 10.1111/j.1348-0421.1996.tb01080.x
DO - 10.1111/j.1348-0421.1996.tb01080.x
M3 - Article
C2 - 8805100
AN - SCOPUS:0029941889
SN - 0385-5600
VL - 40
SP - 359
EP - 363
JO - Microbiology and Immunology
JF - Microbiology and Immunology
IS - 5
ER -
