TY - JOUR
T1 - Evaluation of damage in DNA molecules caused by very-low-frequency magnetic fields using bacterial cells
AU - Haga, Akira
AU - Kumagai, Yoshiaki
AU - Matsuki, Hidetoshi
AU - Endo, Ginro
AU - Igarashi, Akira
AU - Kobayashi, Koichiro
PY - 2005/8
Y1 - 2005/8
N2 - The effect of intermediate frequency magnetic fields or, very-low-frequency magnetic fields (VLFMF) on living biological cells was investigated using a highly sensitive mutagenesis assay method. A bacterial gene expression system for mutation repair (umu system) was used for the sensitive evaluation of damage in DNA molecules. Salmonella typhimurium TA1535 (pSK1002) were exposed to VLFMF (20 kHz and 600 μT) in a specially designed magnetic field loading chamber. The experiment results showed the possibility of applying the umu assay for sensitive and effective evaluation of damage in DNA molecules. No effects from exposure to 20 kHz and 600 μT magnetic fields in terms of damage in DNA molecules were observed.
AB - The effect of intermediate frequency magnetic fields or, very-low-frequency magnetic fields (VLFMF) on living biological cells was investigated using a highly sensitive mutagenesis assay method. A bacterial gene expression system for mutation repair (umu system) was used for the sensitive evaluation of damage in DNA molecules. Salmonella typhimurium TA1535 (pSK1002) were exposed to VLFMF (20 kHz and 600 μT) in a specially designed magnetic field loading chamber. The experiment results showed the possibility of applying the umu assay for sensitive and effective evaluation of damage in DNA molecules. No effects from exposure to 20 kHz and 600 μT magnetic fields in terms of damage in DNA molecules were observed.
KW - LacZ, Y, β-galactosidase
KW - Mutation repairing gene
KW - Salmonella typhimurium TA1535 (pSK1002)
KW - Umu operon
KW - Very-low-frequency magnetic field (VLFMF)
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U2 - 10.1093/ietcom/e88-b.8.3249
DO - 10.1093/ietcom/e88-b.8.3249
M3 - Article
AN - SCOPUS:26044483036
SN - 0916-8516
VL - E88-B
SP - 3249
EP - 3255
JO - IEICE Transactions on Communications
JF - IEICE Transactions on Communications
IS - 8
ER -