TY - JOUR
T1 - Evaluation of genetic diversity of eight grouper species Epinephelus spp. based on microsatellite variations
AU - Koedprang, Worawut
AU - Na-Nakorn, Uthairat
AU - Nakajima, Masamichi
AU - Taniguchi, Nobuhiko
N1 - Funding Information:
We would like to express our gratitude to Thailand Research Fund for the funding of this research through the project entitled “Application of Genetics and Biotechnology for Sustainable Development of Aquaculture” (Senior Research Scholar 2003) awarded to U. Na-Nakorn. W. Koedprang was supported by the Japan Society for Promotion of Science (JSPS) during his work in Tohoku University, Japan. The authors appreciate the comments and checking of English grammar by Dr. Thuy Nguyen, Network of Aquaculture Centres in Asia-Pacific.
PY - 2007/4
Y1 - 2007/4
N2 - Genetic diversity among eight species of grouper, Epinephelus bleekeri, E. coioides, E. malabaricus, E. ongus, E. akaara, E. maculatus, E. merra and E. fuscoguttatus, was studied using six microsatellite loci, Em-01*, Em-03*, Em-07*, Em-08*, Em-10*, and CA-07*, with the aim of exploring the feasibility of using microsatellite data for species identification. The results showed high levels of genetic differentiation among species (FST = 0.4403 and RST = 0.4954). Species identification based on fixed allelic differences was possible between E. coioides, E. ongus, and E. fuscoguttatus at Em-01* and between E. fuscoguttatus and E. ongus at Em-08*. Private alleles were found in all species, except for E. ongus. Pairwise FST ranged 0.238-0.578 (P < 0.008 Bonferroni correction), and Nei's genetic distance ranged 0.433-2.710. Size homoplasy was observed at Em-03*157 allele, which was characterized by a T-C transition at the 119th nucleotide site of PCR products. The genetic assignment test unambiguously assigned each individual to the correct species. Thus, this test can be used for species identification of unknown individuals when the multilocus genotypes of the six microsatellite loci are available. The phylogenetic (neighbor-joining) tree, which was constructed based on the genetic distance matrix, separated the eight grouper species into two main groups.
AB - Genetic diversity among eight species of grouper, Epinephelus bleekeri, E. coioides, E. malabaricus, E. ongus, E. akaara, E. maculatus, E. merra and E. fuscoguttatus, was studied using six microsatellite loci, Em-01*, Em-03*, Em-07*, Em-08*, Em-10*, and CA-07*, with the aim of exploring the feasibility of using microsatellite data for species identification. The results showed high levels of genetic differentiation among species (FST = 0.4403 and RST = 0.4954). Species identification based on fixed allelic differences was possible between E. coioides, E. ongus, and E. fuscoguttatus at Em-01* and between E. fuscoguttatus and E. ongus at Em-08*. Private alleles were found in all species, except for E. ongus. Pairwise FST ranged 0.238-0.578 (P < 0.008 Bonferroni correction), and Nei's genetic distance ranged 0.433-2.710. Size homoplasy was observed at Em-03*157 allele, which was characterized by a T-C transition at the 119th nucleotide site of PCR products. The genetic assignment test unambiguously assigned each individual to the correct species. Thus, this test can be used for species identification of unknown individuals when the multilocus genotypes of the six microsatellite loci are available. The phylogenetic (neighbor-joining) tree, which was constructed based on the genetic distance matrix, separated the eight grouper species into two main groups.
KW - Epinephelus
KW - Genetic diversity
KW - Grouper species
KW - Homoplasy
KW - Microsatellite
UR - http://www.scopus.com/inward/record.url?scp=34147138424&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34147138424&partnerID=8YFLogxK
U2 - 10.1111/j.1444-2906.2007.01328.x
DO - 10.1111/j.1444-2906.2007.01328.x
M3 - Article
AN - SCOPUS:34147138424
SN - 0919-9268
VL - 73
SP - 227
EP - 236
JO - Fisheries Science
JF - Fisheries Science
IS - 2
ER -