Abstract
Six chimeras of the skeletal muscle (RyR1) and cardiac muscle (RyR2) Ca2+ release channels (ryanodine receptors) previously used to identify RyR1 dihydropyridine receptor interactions [Nakai et al. (1998) J. Biol. Chem. 273, 13403] were expressed in HEK293 cells to assess their Ca2+ dependence in [3H]ryanodine binding and single channel measurements. The results indicate that the C-terminal one-fourth has a major role in Ca2+ activation and inactivation of RyR1. Further, our results show that replacement of RyR1 regions with corresponding RyR2 regions can result in loss and/or reduction of [3H]ryanodine binding affinity while maintaining channel activity.
Original language | English |
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Pages (from-to) | 154-158 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 459 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1999 Oct 8 |
Keywords
- Ca release channel
- Cardiac muscle
- HEK293 cell
- Ryanodine receptor
- Skeletal muscle