Evidence for motoneuron lineage-specific regulation of Olig2 in the vertebrate neural tube

Tao Sun; Brian P. Hafler; Sovann Kaing; Masaaki Kitada; Keith L. Ligon; Hans R. Widlund; Dong in Yuk; Charles D. Stiles; David H. Rowitch

doi:10.1016/j.ydbio.2005.12.047

Evidence for motoneuron lineage-specific regulation of Olig2 in the vertebrate neural tube

Tao Sun, Brian P. Hafler, Sovann Kaing, Masaaki Kitada, Keith L. Ligon, Hans R. Widlund, Dong in Yuk, Charles D. Stiles, David H. Rowitch

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Within the motoneuron precursor (pMN) domain of the developing spinal cord, the bHLH transcription factor, Olig2, plays critical roles in pattern formation and the generation of motor neuron and oligodendrocyte precursors. How are the multiple functions of Olig2 regulated? We have isolated a large BAC clone encompassing the human OLIG2 locus that rescues motor neuron and oligodendrocyte development but not normal pattern formation in Olig2^-/- embryos. Within the BAC clone, we identified a conserved 3.6 kb enhancer sub-region that directs reporter expression specifically in the motor neuron lineage but not oligodendrocyte lineage in vivo. Our findings indicate complex regulation of Olig2 by stage- and lineage-specific regulatory elements. They further suggest that transcriptional regulation of Olig2 is involved in segregation of pMN neuroblasts.

Original language	English
Pages (from-to)	152-164
Number of pages	13
Journal	Developmental Biology
Volume	292
Issue number	1
DOIs	https://doi.org/10.1016/j.ydbio.2005.12.047
Publication status	Published - 2006 Apr 1
Externally published	Yes

Keywords

Bacterial Artificial Chromosome (BAC)
Enhancer
Lineage mapping
Motor neuron
Neural tube
Olig2
Oligodendrocyte
Transcription factor
cis-regulation

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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title = "Evidence for motoneuron lineage-specific regulation of Olig2 in the vertebrate neural tube",

abstract = "Within the motoneuron precursor (pMN) domain of the developing spinal cord, the bHLH transcription factor, Olig2, plays critical roles in pattern formation and the generation of motor neuron and oligodendrocyte precursors. How are the multiple functions of Olig2 regulated? We have isolated a large BAC clone encompassing the human OLIG2 locus that rescues motor neuron and oligodendrocyte development but not normal pattern formation in Olig2-/- embryos. Within the BAC clone, we identified a conserved 3.6 kb enhancer sub-region that directs reporter expression specifically in the motor neuron lineage but not oligodendrocyte lineage in vivo. Our findings indicate complex regulation of Olig2 by stage- and lineage-specific regulatory elements. They further suggest that transcriptional regulation of Olig2 is involved in segregation of pMN neuroblasts.",

keywords = "Bacterial Artificial Chromosome (BAC), Enhancer, Lineage mapping, Motor neuron, Neural tube, Olig2, Oligodendrocyte, Transcription factor, cis-regulation",

author = "Tao Sun and Hafler, {Brian P.} and Sovann Kaing and Masaaki Kitada and Ligon, {Keith L.} and Widlund, {Hans R.} and Yuk, {Dong in} and Stiles, {Charles D.} and Rowitch, {David H.}",

note = "Funding Information: The authors wish to thank Qiufu Ma, Paul Gray and David Anderson for comments and stimulating discussions. T.S. is grateful to the National Multiple Sclerosis Society for an Advanced Postdoctoral Fellowship and acknowledges Chris Walsh for advice and support during the course of this work. These studies were funded by grants from the NIH to D.H.R. (NS4051) and C.D.S. (NS047572), and a Collaborative Center Grant from the National Multiple Sclerosis Society. ",

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doi = "10.1016/j.ydbio.2005.12.047",

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AU - Sun, Tao

AU - Hafler, Brian P.

AU - Kaing, Sovann

AU - Kitada, Masaaki

AU - Ligon, Keith L.

AU - Widlund, Hans R.

AU - Yuk, Dong in

AU - Stiles, Charles D.

AU - Rowitch, David H.

N1 - Funding Information: The authors wish to thank Qiufu Ma, Paul Gray and David Anderson for comments and stimulating discussions. T.S. is grateful to the National Multiple Sclerosis Society for an Advanced Postdoctoral Fellowship and acknowledges Chris Walsh for advice and support during the course of this work. These studies were funded by grants from the NIH to D.H.R. (NS4051) and C.D.S. (NS047572), and a Collaborative Center Grant from the National Multiple Sclerosis Society.

PY - 2006/4/1

Y1 - 2006/4/1

N2 - Within the motoneuron precursor (pMN) domain of the developing spinal cord, the bHLH transcription factor, Olig2, plays critical roles in pattern formation and the generation of motor neuron and oligodendrocyte precursors. How are the multiple functions of Olig2 regulated? We have isolated a large BAC clone encompassing the human OLIG2 locus that rescues motor neuron and oligodendrocyte development but not normal pattern formation in Olig2-/- embryos. Within the BAC clone, we identified a conserved 3.6 kb enhancer sub-region that directs reporter expression specifically in the motor neuron lineage but not oligodendrocyte lineage in vivo. Our findings indicate complex regulation of Olig2 by stage- and lineage-specific regulatory elements. They further suggest that transcriptional regulation of Olig2 is involved in segregation of pMN neuroblasts.

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Evidence for motoneuron lineage-specific regulation of Olig2 in the vertebrate neural tube

Abstract

Keywords

ASJC Scopus subject areas

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