TY - JOUR
T1 - Evidence of biomembrane lipid glycation
AU - Miyazawa, Teruo
AU - Shoji, Naoki
AU - Nakagawa, Kiyotaka
PY - 2006
Y1 - 2006
N2 - Although Maillard reaction (glycation) between protein and glucose has been thoroughly investigated, we have found that it. is possible for glycation to occur between lipid and glucose. Our finding hypothesizes that, under a hyperglycemic condition (i.e. diabetes), membrane phospholipid may be abnormally glycated in vivo, contributing to pathogenesis. In evaluating the hypothesis, we demonstrated that, glycated phospholipid caused oxidative stress (lipid peroxidation) and angiogenesis, and developed a preparation method of pure glycated phospholipid as well as its highly sensitive assay. In this paper, we introduce our analysis technique of glycated phospholipid, give evidence of the occurrence of lipid glycation in a biomembrane, and discuss the relation of lipid glycation to lipid peroxidation and cell functional disorder.
AB - Although Maillard reaction (glycation) between protein and glucose has been thoroughly investigated, we have found that it. is possible for glycation to occur between lipid and glucose. Our finding hypothesizes that, under a hyperglycemic condition (i.e. diabetes), membrane phospholipid may be abnormally glycated in vivo, contributing to pathogenesis. In evaluating the hypothesis, we demonstrated that, glycated phospholipid caused oxidative stress (lipid peroxidation) and angiogenesis, and developed a preparation method of pure glycated phospholipid as well as its highly sensitive assay. In this paper, we introduce our analysis technique of glycated phospholipid, give evidence of the occurrence of lipid glycation in a biomembrane, and discuss the relation of lipid glycation to lipid peroxidation and cell functional disorder.
KW - Amadori-PE
KW - Diabetes
KW - Linear ion-trap LC/MS/MS
KW - Lipid glycation
KW - Lipid peroxidation
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U2 - 10.2116/bunsekikagaku.55.907
DO - 10.2116/bunsekikagaku.55.907
M3 - Article
AN - SCOPUS:33845748096
SN - 0525-1931
VL - 55
SP - 907
EP - 917
JO - Bunseki Kagaku
JF - Bunseki Kagaku
IS - 12
ER -