Evolving catalytic antibodies in a phage-displayed combinatorial library

Ikuo Fuj'Ii, Shiro Fukuyama, Yoshiharu Iwabuchi, Ryuji Tanimura

Research output: Contribution to journalArticlepeer-review

72 Citations (Scopus)


In vitro affinity maturation for evolving catalytic antibodies has been demonstrated by generating a diverse repertoire of the appropriate complementarity-determining regions on a phage surface. Phage display is followed by a selection based on binding to an altered antigen that was not used at the time of immunization, and provides variants with new catalytic activity and substrate specificity. This library format reduces the time needed to isolate the desired catalytic antibody fragments to under 2 weeks.

Original languageEnglish
Pages (from-to)463-467
Number of pages5
JournalNature Biotechnology
Issue number5
Publication statusPublished - 1998 May
Externally publishedYes


  • Molecular evolution
  • Protein engineering
  • Transition state

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering


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