Exchange of glutamate and γ-aminobutyrate in a Lactobacillus strain

Takeshi Higuchi, Hisanobu Hayashi, Keietsu Abe

Research output: Contribution to journalArticlepeer-review

149 Citations (Scopus)


Lactobacillus sp. strain E1 catalyzed the decarboxylation of glutamate (Glu), resulting in a nearly stoichiometric release of the products γ- aminobutyrate (GABA) and CO2. This decarboxylation was associated with the net synthesis of ATP. ATP synthesis was inhibited almost completely by nigericin and about 70% by N,N'-dicyclohexylcarbodiimide (DCCD), without inhibition of the decarboxylation. These findings are consistent with the possibility that a proton motive force arises from the cytoplasmic proton consumption that accompanies glutamate decarboxylation and the electrogenic Glu/GABA antiporter and the possibility that this proton motive force is coupled with ATP synthesis by DCCD-sensitive ATPase.

Original languageEnglish
Pages (from-to)3362-3364
Number of pages3
JournalJournal of bacteriology
Issue number10
Publication statusPublished - 1997 May
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology


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