Exercise training upregulates nitric oxide synthases in the kidney of rats with chronic heart failure

Daisuke Ito, Osamu Ito, Nobuyoshi Mori, Pengyu Cao, Chihiro Suda, Yoshikazu Muroya, Kiyotaka Hao, Hiroaki Shimokawa, Masahiro Kohzuki

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Summary: There is an interaction between heart and kidney diseases, which is a condition termed cardiorenal syndrome. Exercise training has cardioprotective effects, involving upregulation of endothelial (e) nitric oxide synthase (NOS) in the cardiovascular system. However, the effects of exercise training on NOS in the kidney with heart disease are unknown. The aim of the present study was to investigate whether exercise training upregulates NOS in the kidney, left ventricle and aorta of rats with chronic heart failure (CHF). Male Sprague-Dawley rats underwent left coronary artery ligation (LCAL) to induce CHF and were randomly assigned to sedentary or treadmill exercise groups 4 weeks after LCAL. Three days after exercising for 4 weeks, urine samples were collected for 24 h and blood samples were collected following decapitation. Nitric oxide synthase activity and protein expression were examined. Significant interactions between CHF and exercise training were observed on parameters of cardiac and renal function. Exercise training improved cardiac function, decreased plasma B-type natriuretic peptide levels, decreased urinary albumin excretion and increased creatinine clearance in CHF rats. Nitric oxide synthase activity, eNOS expression and neuronal (n) NOS expression were significantly decreased in the left ventricle and kidney of CHF rats. Exercise training significantly increased NOS activity and eNOS and nNOS expression. Upregulation of NOS in the kidney and left ventricle may contribute, in part, to the renal and cardiac protective effects of exercise training in cardiorenal syndrome in CHF rats.

Original languageEnglish
Pages (from-to)617-625
Number of pages9
JournalClinical and Experimental Pharmacology and Physiology
Issue number9
Publication statusPublished - 2013 Sept


  • Chronic heart failure
  • Exercise
  • Kidney
  • Nitric oxide synthase


Dive into the research topics of 'Exercise training upregulates nitric oxide synthases in the kidney of rats with chronic heart failure'. Together they form a unique fingerprint.

Cite this