TY - JOUR
T1 - Expansion of triplex recognition codes by the use of novel bicyclic nucleoside derivatives (WNA)
AU - Taniguchi, Yosuke
AU - Nakamura, Ayako
AU - Senko, Yusuke
AU - Kodama, Keiichi
AU - Nagatsugi, Fumi
AU - Sasaki, Shigeki
N1 - Funding Information:
Accepted February 2005. *Selective formation of stable triplexes including a TA or a CG interrupting site with new bicyclic nucleoside analogs (WNA).[2] This work was supported by Grant-in-Aid for Scientific (B) from Japan Society for the Promotion of Science (JSPS), CREST from Japan Society and Technology Corporation, and Advanced and Innovational Research Program in Life Sciences from the Ministry of Education, Culture, Sports, Science and Technology, the Japanese Government. Address correspondence to Shigeki Sasaki, CREST, Japan Science and Technology Corporation, Japan; Fax: +81-92-642-6876; E-mail: sasaki@phar.kyushu-u.ac.jp
PY - 2005
Y1 - 2005
N2 - Recently, we have developed new base analogs (WNA) and demonstrated that WNA-T538;T with thymine and WNA-C with cytosine stabilize non-natural antiparallel triplexes with a TA or a CG interrupting site, respectively. However, limitations in recognizable sequences with the WNA-containing TFO were also found. The objective of this study is to search better WNA analogs for expansion of triplex recognition codes to general duplex sequences. In this study, we designed new WNA analogs by systematic modification of the aromatic part and the recognition part. The new WNA analogs with the benzene ring substituted with bromide or cyanide have determined for selective stabilization of triplexes at a TA interrupting site, and general formation of triplexes having a TA interrupting site has been achieved.
AB - Recently, we have developed new base analogs (WNA) and demonstrated that WNA-T538;T with thymine and WNA-C with cytosine stabilize non-natural antiparallel triplexes with a TA or a CG interrupting site, respectively. However, limitations in recognizable sequences with the WNA-containing TFO were also found. The objective of this study is to search better WNA analogs for expansion of triplex recognition codes to general duplex sequences. In this study, we designed new WNA analogs by systematic modification of the aromatic part and the recognition part. The new WNA analogs with the benzene ring substituted with bromide or cyanide have determined for selective stabilization of triplexes at a TA interrupting site, and general formation of triplexes having a TA interrupting site has been achieved.
KW - Antigene
KW - Interrupting Site
KW - Molecular Recognition
KW - Non-Natural Nucleoside
KW - Triplex DNA
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U2 - 10.1081/NCN-200060309
DO - 10.1081/NCN-200060309
M3 - Article
C2 - 16250103
AN - SCOPUS:26644461460
SN - 1525-7770
VL - 24
SP - 823
EP - 827
JO - Nucleosides, Nucleotides and Nucleic Acids
JF - Nucleosides, Nucleotides and Nucleic Acids
IS - 5-7
ER -