TY - JOUR
T1 - Expression and functional analyses of five B-class genes in the grape hyacinth (Muscari armeniacum)
AU - Miura, Kana
AU - Nakada, Mutsumi
AU - Kubota, Shosei
AU - Sato, Shusei
AU - Nagano, Soichiro
AU - Kobayashi, Akie
AU - Teranishi, Mika
AU - Nakano, Masaru
AU - Kanno, Akira
N1 - Funding Information:
This work was partly supported by JSPS KAKENHI Grant Number (16380024).
Publisher Copyright:
© 2019 The Japanese Society for Horticultural Science (JSHS), All rights reserved.
PY - 2019
Y1 - 2019
N2 - The modified ABC model explains the floral morphology of many monocots, such as the lily and tulip, in which the perianth consists of two layers of almost identical petaloid tepals. According to the modified ABC model, B-class genes are expressed in two perianth whorls, inducing the petaloid structure in both whorls 1 and 2. In this study, we analyzed the expression and function of the B-class genes in the grape hyacinth (Muscari armeniacum). We isolated two DEFICIENS (DEF)-like genes (MaDEF1 and MaDEF2) and three GLOBOSA (GLO)-like genes (MaGLOA1, MaGLOA2, and MaGLOB) from M. armeniacum using rapid amplification of cDNA ends (RACE). Expression analysis showed that MaDEF1 and MaDEF2 were expressed in whorls 1, 2, and 3, whereas MaGLOA1, MaGLOA2, and MaGLOB were expressed in all four whorls. These results support the modified ABC model in M. armeniacum. Overexpression of MaGLOA1 and MaGLOB in Arabidopsis thaliana resulted in a morphological change of sepals to petaloid structures in whorl 1, indicating that the function of these genes is similar that of the B-class orthologs PISTILLATA and GLO in A. thaliana and Antirrhinum majus, respectively. In addition, yeast two-hybrid assays revealed strong protein–protein interactions between MaDEF1 and MaGLOA1, suggesting that MaDEF1–MaGLOA1 is likely to have the main B-function in M. armeniacum. These data support the modified ABC model in M. armeniacum.
AB - The modified ABC model explains the floral morphology of many monocots, such as the lily and tulip, in which the perianth consists of two layers of almost identical petaloid tepals. According to the modified ABC model, B-class genes are expressed in two perianth whorls, inducing the petaloid structure in both whorls 1 and 2. In this study, we analyzed the expression and function of the B-class genes in the grape hyacinth (Muscari armeniacum). We isolated two DEFICIENS (DEF)-like genes (MaDEF1 and MaDEF2) and three GLOBOSA (GLO)-like genes (MaGLOA1, MaGLOA2, and MaGLOB) from M. armeniacum using rapid amplification of cDNA ends (RACE). Expression analysis showed that MaDEF1 and MaDEF2 were expressed in whorls 1, 2, and 3, whereas MaGLOA1, MaGLOA2, and MaGLOB were expressed in all four whorls. These results support the modified ABC model in M. armeniacum. Overexpression of MaGLOA1 and MaGLOB in Arabidopsis thaliana resulted in a morphological change of sepals to petaloid structures in whorl 1, indicating that the function of these genes is similar that of the B-class orthologs PISTILLATA and GLO in A. thaliana and Antirrhinum majus, respectively. In addition, yeast two-hybrid assays revealed strong protein–protein interactions between MaDEF1 and MaGLOA1, suggesting that MaDEF1–MaGLOA1 is likely to have the main B-function in M. armeniacum. These data support the modified ABC model in M. armeniacum.
KW - DEFICIENS-like gene
KW - GLOBOSA-like gene
KW - MADS-box gene
KW - Real-time PCR
KW - Yeast two-hybrid assay
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U2 - 10.2503/hortj.UTD-036
DO - 10.2503/hortj.UTD-036
M3 - Article
AN - SCOPUS:85065503263
SN - 2189-0102
VL - 88
SP - 284
EP - 292
JO - Horticulture Journal
JF - Horticulture Journal
IS - 2
ER -