Expression and localization of regenerating gene I in a rat liver regeneration model

Jingshu Wang, Souichi Koyota, Xiaoping Zhou, Yasuharu Ueno, Li Ma, Masami Kawagoe, Yukio Koizumi, Hiroshi Okamoto, Toshihiro Sugiyama

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Regenerating gene (Reg) I has been identified as a regenerative/proliferative factor for pancreatic islet cells. We examined Reg I expression in the regenerating liver of a rat model that had been administered 2-acetylaminofluorene and treated with 70% partial hepatectomy (2-AAF/PH model), where hepatocyte and cholangiocyte proliferation was suppressed and the hepatic stem cells and/or hepatic progenitor cells were activated. In a detailed time course study of activation of hepatic stem cells in the 2-AAF/PH model, utilizing immunofluorescence staining with antibodies of Reg I and other cell-type-specific markers, we found that Reg I-expressing cells are present in the bile ductules and increased during regeneration. Reg I-expressing cells were colocalized with CK19, OV6, and AFP. These results demonstrate that Reg I is significantly upregulated in the liver of the 2-AAF/PH rat model, accompanied by the formation of bile ductules during liver regeneration.

Original languageEnglish
Pages (from-to)472-477
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - 2009 Mar 13


  • 2-AAF/PH
  • Bile ducts
  • Hepatic progenitor cell
  • Hepatic stem cell
  • Liver regeneration
  • Oval cell
  • Partial hepatectomy
  • Regenerating gene


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