TY - JOUR
T1 - Expression of cementum-derived attachment protein in bovine tooth germ during cementogenesis
AU - Saito, M.
AU - Iwase, M.
AU - Maslan, S.
AU - Nozaki, N.
AU - Yamauchi, M.
AU - Handa, K.
AU - Takahashi, O.
AU - Sato, S.
AU - Kawase, T.
AU - Teranaka, T.
AU - Narayanan, A. S.
N1 - Funding Information:
The authors are grateful to Dr. Shigeaki Kurata and Dr. Hideyuki Negishi for technical assistance of with the cell culture and affinity chromatography. We also thank Dr. Motoshi Suzuki for advice and discussions during the course of this work. Parts of this study were supported by NIH Grants DE-10491 and DE-08229.
PY - 2001
Y1 - 2001
N2 - Cementum-derived attachment protein (CAP) is a 56 kDa collagenous protein that promotes attachment of mesenchymal cells. Previous studies have shown that the presence of CAP is restricted to cementum in adult human tissues. In this study, we report generation of a monoclonal antibody against CAP and its use for the investigation of CAP in developing bovine tooth germs. Mice were immunized with CAP purified from bovine cementum, and a monoclonal antibody, 3G9, was produced. Immunohistochemical staining of bovine tooth germ at root forming stage using 3G9 antibody showed that the tissue distribution of CAP expression was limited to cementum matrix and cementoblasts during cementogenesis. Alveolar bone did not stain with the 3G9 antibody, whereas anti-type I collagen stained positively. CAP was purified from bovine tooth germs with immunoaffinity purification using the 3G9 antibody. Examination of the immunoaffinity-purified fraction showed that CAP existed in tooth germ as a 65 kDa protein. The protein was susceptible to bacterial collagenase. To investigate the possible biological function of CAP during cementogenesis, we isolated dental follicle cells from the bovine tooth germ, and showed that they adhered to surfaces containing CAP. These data demonstrate that CAP is expressed by bovine cementoblasts as a 65 kDa protein and that the CAP may have a function in cementogenesis.
AB - Cementum-derived attachment protein (CAP) is a 56 kDa collagenous protein that promotes attachment of mesenchymal cells. Previous studies have shown that the presence of CAP is restricted to cementum in adult human tissues. In this study, we report generation of a monoclonal antibody against CAP and its use for the investigation of CAP in developing bovine tooth germs. Mice were immunized with CAP purified from bovine cementum, and a monoclonal antibody, 3G9, was produced. Immunohistochemical staining of bovine tooth germ at root forming stage using 3G9 antibody showed that the tissue distribution of CAP expression was limited to cementum matrix and cementoblasts during cementogenesis. Alveolar bone did not stain with the 3G9 antibody, whereas anti-type I collagen stained positively. CAP was purified from bovine tooth germs with immunoaffinity purification using the 3G9 antibody. Examination of the immunoaffinity-purified fraction showed that CAP existed in tooth germ as a 65 kDa protein. The protein was susceptible to bacterial collagenase. To investigate the possible biological function of CAP during cementogenesis, we isolated dental follicle cells from the bovine tooth germ, and showed that they adhered to surfaces containing CAP. These data demonstrate that CAP is expressed by bovine cementoblasts as a 65 kDa protein and that the CAP may have a function in cementogenesis.
KW - Antibody to attachment protein
KW - Calcification
KW - Cementoblasts
KW - Cementum-derived attachment protein
KW - Dental follicle cells
KW - Differentiation
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U2 - 10.1016/S8756-3282(01)00573-7
DO - 10.1016/S8756-3282(01)00573-7
M3 - Article
C2 - 11557368
AN - SCOPUS:0034846420
SN - 8756-3282
VL - 29
SP - 242
EP - 248
JO - Bone
JF - Bone
IS - 3
ER -
