TY - JOUR
T1 - Expression of genes involved in repair of DNA double-strand breaks in normal and tumor tissues
AU - Sakata, Koh Ichi
AU - Matsumoto, Yoshihisa
AU - Tauchi, Hiroshi
AU - Satoh, Masaaki
AU - Oouchi, Atushi
AU - Nagakura, Hisayasu
AU - Koito, Kazumitsu
AU - Hosoi, Yoshio
AU - Suzuki, Norio
AU - Komatsu, Kenshi
AU - Hareyama, Masato
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2001/1/1
Y1 - 2001/1/1
N2 - Background: DNA double-strand breaks (DSB) are the major lethal lesions induced by ionizing radiation. The capability for DNA DSB repair is crucial for inherent radiosensitivity of tumor and normal cells. DNA-PKcs, Ku 70, Ku 85, Xrcc4, and Nbs1 play a critical role in DNA DSB repair. Methods: We immunohistochemically investigated the expression of DNA-PKcs, Ku 70, Ku85, Xrcc4, and Nbs1 in 134 specimens from various normal and tumor tissues with different radiosensitivity. Results and Conclusion: Immunopositivity to Ku70, Ku85, DNA-PKcs, Xrcc4, and Nbs1 was found in all tumor tissues examined. The staining for Ku70, Ku85, and DNA-PKcs was nuclear; but, for Xrcc4 and Nbs1, it was nuclear and cytoplasmic. There were no apparent differences in the expression of these five proteins among cancerous tissues and the corresponding normal tissues. No apparent differences in nuclear staining intensity were detected in the expression of these five proteins among tumor tissues with different radiosensitivity, although non-Hodgkins' lymphoma (B or T cell) tended to show a lower expression than the others. The stromal cells generally expressed these five proteins at much lower frequency than either tumor or epithelial cells in both tumor and normal tissues.
AB - Background: DNA double-strand breaks (DSB) are the major lethal lesions induced by ionizing radiation. The capability for DNA DSB repair is crucial for inherent radiosensitivity of tumor and normal cells. DNA-PKcs, Ku 70, Ku 85, Xrcc4, and Nbs1 play a critical role in DNA DSB repair. Methods: We immunohistochemically investigated the expression of DNA-PKcs, Ku 70, Ku85, Xrcc4, and Nbs1 in 134 specimens from various normal and tumor tissues with different radiosensitivity. Results and Conclusion: Immunopositivity to Ku70, Ku85, DNA-PKcs, Xrcc4, and Nbs1 was found in all tumor tissues examined. The staining for Ku70, Ku85, and DNA-PKcs was nuclear; but, for Xrcc4 and Nbs1, it was nuclear and cytoplasmic. There were no apparent differences in the expression of these five proteins among cancerous tissues and the corresponding normal tissues. No apparent differences in nuclear staining intensity were detected in the expression of these five proteins among tumor tissues with different radiosensitivity, although non-Hodgkins' lymphoma (B or T cell) tended to show a lower expression than the others. The stromal cells generally expressed these five proteins at much lower frequency than either tumor or epithelial cells in both tumor and normal tissues.
KW - Cancer
KW - DNA double-strand breaks
KW - DNA repair
KW - Immunohistochemistry
KW - Radiosensitivity
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U2 - 10.1016/S0360-3016(00)01352-3
DO - 10.1016/S0360-3016(00)01352-3
M3 - Article
C2 - 11163510
AN - SCOPUS:0035160401
SN - 0360-3016
VL - 49
SP - 161
EP - 167
JO - International Journal of Radiation Oncology Biology Physics
JF - International Journal of Radiation Oncology Biology Physics
IS - 1
ER -
