Expression of highly sulfated keratan sulfate synthesized in human glioblastoma cells

Norihito Hayatsu; Satoshi Ogasawara; Mika Kato Kaneko; Yukinari Kato; Hisashi Narimatsu

doi:10.1016/j.bbrc.2008.01.058

Expression of highly sulfated keratan sulfate synthesized in human glioblastoma cells

Norihito Hayatsu, Satoshi Ogasawara, Mika Kato Kaneko, Yukinari Kato, Hisashi Narimatsu

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Keratan sulfate (KS) proteoglycan is expressed in the extracellular matrix or cell surface in numerous tissues, predominantly in those of the cornea, cartilage, and brain. However, its structure, function, and regulation remain poorly understood. Our investigation of KS expression in glioblastoma cell lines using Western-blot and flow cytometry with anti-KS antibody (5D4) revealed that LN229 glioblastoma cell highly expresses KS on a cell surface. Real-time PCR analysis showed that LN229 expresses a high level of keratan sulfate Gal-6-sulfotransferase. Results of this study also demonstrate that recombinant 5D4-reactive aggrecan is produced in LN229. Taken together, these results suggest that LN229 produces 5D4-reactive highly sulfated KS and is useful to investigate the KS structure and function in glioblastoma.

Original language	English
Pages (from-to)	217-222
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	368
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2008.01.058
Publication status	Published - 2008 Apr 4
Externally published	Yes

Keywords

Glioblastoma
Glycogene
Keratan sulfate
Proteoglycan

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2008.01.058

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title = "Expression of highly sulfated keratan sulfate synthesized in human glioblastoma cells",

abstract = "Keratan sulfate (KS) proteoglycan is expressed in the extracellular matrix or cell surface in numerous tissues, predominantly in those of the cornea, cartilage, and brain. However, its structure, function, and regulation remain poorly understood. Our investigation of KS expression in glioblastoma cell lines using Western-blot and flow cytometry with anti-KS antibody (5D4) revealed that LN229 glioblastoma cell highly expresses KS on a cell surface. Real-time PCR analysis showed that LN229 expresses a high level of keratan sulfate Gal-6-sulfotransferase. Results of this study also demonstrate that recombinant 5D4-reactive aggrecan is produced in LN229. Taken together, these results suggest that LN229 produces 5D4-reactive highly sulfated KS and is useful to investigate the KS structure and function in glioblastoma.",

keywords = "Glioblastoma, Glycogene, Keratan sulfate, Proteoglycan",

author = "Norihito Hayatsu and Satoshi Ogasawara and Kaneko, {Mika Kato} and Yukinari Kato and Hisashi Narimatsu",

note = "Funding Information: We thank Ms. Nana Matsuura for technical assistance and Dr. Hiromichi Sawaki for helpful discussion. We are very grateful to Drs. Webster K. Cavenee and Kazuhiko Mishima for generous donation of materials. This study was supported in part by New Energy and Industrial Technology Development Organization (NEDO), by the Osaka Cancer Research Foundation (MK. Kaneko), by Mitsubishi Pharma Research Foundation (Y. Kato), by the YASUDA Medical Foundation (Y. Kato), and by the Inoue Foundation for Science (Y. Kato).",

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doi = "10.1016/j.bbrc.2008.01.058",

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AU - Hayatsu, Norihito

AU - Ogasawara, Satoshi

AU - Kaneko, Mika Kato

AU - Kato, Yukinari

AU - Narimatsu, Hisashi

N1 - Funding Information: We thank Ms. Nana Matsuura for technical assistance and Dr. Hiromichi Sawaki for helpful discussion. We are very grateful to Drs. Webster K. Cavenee and Kazuhiko Mishima for generous donation of materials. This study was supported in part by New Energy and Industrial Technology Development Organization (NEDO), by the Osaka Cancer Research Foundation (MK. Kaneko), by Mitsubishi Pharma Research Foundation (Y. Kato), by the YASUDA Medical Foundation (Y. Kato), and by the Inoue Foundation for Science (Y. Kato).

PY - 2008/4/4

Y1 - 2008/4/4

N2 - Keratan sulfate (KS) proteoglycan is expressed in the extracellular matrix or cell surface in numerous tissues, predominantly in those of the cornea, cartilage, and brain. However, its structure, function, and regulation remain poorly understood. Our investigation of KS expression in glioblastoma cell lines using Western-blot and flow cytometry with anti-KS antibody (5D4) revealed that LN229 glioblastoma cell highly expresses KS on a cell surface. Real-time PCR analysis showed that LN229 expresses a high level of keratan sulfate Gal-6-sulfotransferase. Results of this study also demonstrate that recombinant 5D4-reactive aggrecan is produced in LN229. Taken together, these results suggest that LN229 produces 5D4-reactive highly sulfated KS and is useful to investigate the KS structure and function in glioblastoma.

AB - Keratan sulfate (KS) proteoglycan is expressed in the extracellular matrix or cell surface in numerous tissues, predominantly in those of the cornea, cartilage, and brain. However, its structure, function, and regulation remain poorly understood. Our investigation of KS expression in glioblastoma cell lines using Western-blot and flow cytometry with anti-KS antibody (5D4) revealed that LN229 glioblastoma cell highly expresses KS on a cell surface. Real-time PCR analysis showed that LN229 expresses a high level of keratan sulfate Gal-6-sulfotransferase. Results of this study also demonstrate that recombinant 5D4-reactive aggrecan is produced in LN229. Taken together, these results suggest that LN229 produces 5D4-reactive highly sulfated KS and is useful to investigate the KS structure and function in glioblastoma.

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KW - Glycogene

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KW - Proteoglycan

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Expression of highly sulfated keratan sulfate synthesized in human glioblastoma cells

Abstract

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