TY - JOUR
T1 - Expression of mRNA encoding IFNα in macrophages stimulated with Lactobacillus gasseri
AU - Kitazawa, Haruki
AU - Tomioka, Yoshihisa
AU - Matsumura, Kazuki
AU - Aso, Hisashi
AU - Mizugaki, Michinao
AU - Itoh, Takatoshi
AU - Yamaguchi, Takahiro
N1 - Funding Information:
This work was partially supported by a Grant-in-Aid for encouragement of young scientists (Number 05760201) from the Ministry of Education, Science and Culture of Japan to Dr. H. Kitazawa.
PY - 1994/7/15
Y1 - 1994/7/15
N2 - The ability of Lactobacillus gasseri, a dairy lactic acid bacterium, to induce interferon (IFN) was investigated in murine macrophage cultures. IFNα was substantially induced by some strais of L. gasseri and the titers were the highest at a concentration of 100 μg ml-1 of L. gasseri DSM20243T. The expression of mRNA encoding IFNα was detected in spleen-macrophages (SP-Mθ) and Peyer's patch-adherent cells stimulated with L. gasseri DSM20243T. Actinomycin D and cycloheximide added to SP-Mθ cultures showed that the mRNA was synthesized by 0.5 h, and that IFNα was produced within 3 to 6 h after the stimulation with L. gasseri DSM20243T. The results support the notion that dairy products containing L. gasseri can be 'physiologically functional foods'.
AB - The ability of Lactobacillus gasseri, a dairy lactic acid bacterium, to induce interferon (IFN) was investigated in murine macrophage cultures. IFNα was substantially induced by some strais of L. gasseri and the titers were the highest at a concentration of 100 μg ml-1 of L. gasseri DSM20243T. The expression of mRNA encoding IFNα was detected in spleen-macrophages (SP-Mθ) and Peyer's patch-adherent cells stimulated with L. gasseri DSM20243T. Actinomycin D and cycloheximide added to SP-Mθ cultures showed that the mRNA was synthesized by 0.5 h, and that IFNα was produced within 3 to 6 h after the stimulation with L. gasseri DSM20243T. The results support the notion that dairy products containing L. gasseri can be 'physiologically functional foods'.
KW - IFNα-mRNA expression
KW - Lactobacillus gasseri
KW - Murine macrophage
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U2 - 10.1111/j.1574-6968.1994.tb07052.x
DO - 10.1111/j.1574-6968.1994.tb07052.x
M3 - Article
C2 - 8076807
AN - SCOPUS:0028363905
SN - 0378-1097
VL - 120
SP - 315
EP - 321
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 3
ER -