Expression of Reg/PAP family members during motor nerve regeneration in rat

Kazuhiko Namikawa, Munehisa Fukushima, Koichi Murakami, Akinobu Suzuki, Shin Takasawa, Hiroshi Okamoto, Hiroshi Kiyama

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)


In this study, we examined the expression of mRNAs for Regenerating gene (Reg)/pancreatitis-associated protein (PAP) family members following hypoglossal nerve injury in rats. In addition to four rat family members (RegI, Reg-2/PAP I, PAP II, and PAP III) that had been identified, we newly cloned and sequenced a type-IV Reg gene in rats. Among these five family members, the expression of Reg-2/PAP I mRNA was predominantly enhanced in injured motor neurons after axotomy. Furthermore, a marked induction of PAP III mRNA was observed in the distal part of the injured nerve. A polyclonal antibody was raised against PAP III, and a Western blotting analysis using this antibody confirmed an increased level of PAP III protein in the injured nerve. These results suggest that Reg family members would be new mediators among injured neurons and glial cells, and may play pivotal roles during nerve regeneration.

Original languageEnglish
Pages (from-to)126-134
Number of pages9
JournalBiochemical and biophysical research communications
Issue number1
Publication statusPublished - 2005 Jun 24


  • HIP
  • Inflammation
  • Motor neuron
  • Nerve regeneration
  • PAP
  • Reg family
  • Schwann cell

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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