Expression of Reg/PAP family members during motor nerve regeneration in rat

Kazuhiko Namikawa; Munehisa Fukushima; Koichi Murakami; Akinobu Suzuki; Shin Takasawa; Hiroshi Okamoto; Hiroshi Kiyama

doi:10.1016/j.bbrc.2005.04.105

Expression of Reg/PAP family members during motor nerve regeneration in rat

Kazuhiko Namikawa, Munehisa Fukushima, Koichi Murakami, Akinobu Suzuki, Shin Takasawa, Hiroshi Okamoto, Hiroshi Kiyama

Surgery

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Abstract

In this study, we examined the expression of mRNAs for Regenerating gene (Reg)/pancreatitis-associated protein (PAP) family members following hypoglossal nerve injury in rats. In addition to four rat family members (RegI, Reg-2/PAP I, PAP II, and PAP III) that had been identified, we newly cloned and sequenced a type-IV Reg gene in rats. Among these five family members, the expression of Reg-2/PAP I mRNA was predominantly enhanced in injured motor neurons after axotomy. Furthermore, a marked induction of PAP III mRNA was observed in the distal part of the injured nerve. A polyclonal antibody was raised against PAP III, and a Western blotting analysis using this antibody confirmed an increased level of PAP III protein in the injured nerve. These results suggest that Reg family members would be new mediators among injured neurons and glial cells, and may play pivotal roles during nerve regeneration.

Original language	English
Pages (from-to)	126-134
Number of pages	9
Journal	Biochemical and biophysical research communications
Volume	332
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2005.04.105
Publication status	Published - 2005 Jun 24

Keywords

HIP
Inflammation
Motor neuron
Nerve regeneration
PAP
Reg family
Schwann cell

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2005.04.105

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title = "Expression of Reg/PAP family members during motor nerve regeneration in rat",

abstract = "In this study, we examined the expression of mRNAs for Regenerating gene (Reg)/pancreatitis-associated protein (PAP) family members following hypoglossal nerve injury in rats. In addition to four rat family members (RegI, Reg-2/PAP I, PAP II, and PAP III) that had been identified, we newly cloned and sequenced a type-IV Reg gene in rats. Among these five family members, the expression of Reg-2/PAP I mRNA was predominantly enhanced in injured motor neurons after axotomy. Furthermore, a marked induction of PAP III mRNA was observed in the distal part of the injured nerve. A polyclonal antibody was raised against PAP III, and a Western blotting analysis using this antibody confirmed an increased level of PAP III protein in the injured nerve. These results suggest that Reg family members would be new mediators among injured neurons and glial cells, and may play pivotal roles during nerve regeneration.",

keywords = "HIP, Inflammation, Motor neuron, Nerve regeneration, PAP, Reg family, Schwann cell",

author = "Kazuhiko Namikawa and Munehisa Fukushima and Koichi Murakami and Akinobu Suzuki and Shin Takasawa and Hiroshi Okamoto and Hiroshi Kiyama",

note = "Funding Information: We thank Dr. Graf (University Hospital, Z{\"u}rich) for donating pPIC9-PAP III and Dr. Henderson (Developmental Biology Institute, Marseille) for donating the hybridoma producing the anti-p75 antibody. We are also grateful to C. Kadono, I. Jikihara, and T. Iwasaki for technical assistance, and E. Fukui and T. Kawai for secretarial assistance. This study was supported in part by grants from the Ministry of Health, Labor and Welfare of Japan, the Ministry of Education, Science, Technology, Sports and Culture, and the Japan Science and Technology Corporation.",

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AU - Namikawa, Kazuhiko

AU - Fukushima, Munehisa

AU - Murakami, Koichi

AU - Suzuki, Akinobu

AU - Takasawa, Shin

AU - Okamoto, Hiroshi

AU - Kiyama, Hiroshi

N1 - Funding Information: We thank Dr. Graf (University Hospital, Zürich) for donating pPIC9-PAP III and Dr. Henderson (Developmental Biology Institute, Marseille) for donating the hybridoma producing the anti-p75 antibody. We are also grateful to C. Kadono, I. Jikihara, and T. Iwasaki for technical assistance, and E. Fukui and T. Kawai for secretarial assistance. This study was supported in part by grants from the Ministry of Health, Labor and Welfare of Japan, the Ministry of Education, Science, Technology, Sports and Culture, and the Japan Science and Technology Corporation.

PY - 2005/6/24

Y1 - 2005/6/24

N2 - In this study, we examined the expression of mRNAs for Regenerating gene (Reg)/pancreatitis-associated protein (PAP) family members following hypoglossal nerve injury in rats. In addition to four rat family members (RegI, Reg-2/PAP I, PAP II, and PAP III) that had been identified, we newly cloned and sequenced a type-IV Reg gene in rats. Among these five family members, the expression of Reg-2/PAP I mRNA was predominantly enhanced in injured motor neurons after axotomy. Furthermore, a marked induction of PAP III mRNA was observed in the distal part of the injured nerve. A polyclonal antibody was raised against PAP III, and a Western blotting analysis using this antibody confirmed an increased level of PAP III protein in the injured nerve. These results suggest that Reg family members would be new mediators among injured neurons and glial cells, and may play pivotal roles during nerve regeneration.

AB - In this study, we examined the expression of mRNAs for Regenerating gene (Reg)/pancreatitis-associated protein (PAP) family members following hypoglossal nerve injury in rats. In addition to four rat family members (RegI, Reg-2/PAP I, PAP II, and PAP III) that had been identified, we newly cloned and sequenced a type-IV Reg gene in rats. Among these five family members, the expression of Reg-2/PAP I mRNA was predominantly enhanced in injured motor neurons after axotomy. Furthermore, a marked induction of PAP III mRNA was observed in the distal part of the injured nerve. A polyclonal antibody was raised against PAP III, and a Western blotting analysis using this antibody confirmed an increased level of PAP III protein in the injured nerve. These results suggest that Reg family members would be new mediators among injured neurons and glial cells, and may play pivotal roles during nerve regeneration.

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KW - Inflammation

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KW - Nerve regeneration

KW - PAP

KW - Reg family

KW - Schwann cell

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Expression of Reg/PAP family members during motor nerve regeneration in rat

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