TY - JOUR
T1 - Expression of ROCK-1 in human pancreatic cancer
T2 - Its down-regulation by morpholino oligo antisense can reduce the migration of pancreatic cancer cells in vitro
AU - Kaneko, Kenzo
AU - Satoh, Kennichi
AU - Masamune, Atsushi
AU - Satoh, Akihiko
AU - Shimosegawa, Tooru
PY - 2002
Y1 - 2002
N2 - Introduction: Invasion and metastasis of cancer cells require cell motility and adhesion. The small GTPase Rho and one of its effector molecules ROCK regulate cytoskeleton and actomyosin contractility, and play a crucial role in cell adhesion and motility. Results of previous studies showed that the elevated activity of ROCK-1, one of the isomers of ROCK kinases, led to an increase in the activity of invasion and metastasis of cancer cell lines. Aim: To investigate the importance of ROCK-1 in cancer invasion and metastasis. Methodology: We investigated the expression of ROCK-1 in two cancer cell lines and 31 human pancreatic tissues (21 pancreatic cancers [PC] and 10 histologically normal tissues) by immunoblotting and immunohistochemistry. We also examined by haptotaxis assay whether the migratory activity of PC cells could be suppressed by treatment with the morpholino antisense oligonucleotide in vitro. Results: The expression of ROCK-1 was found in 18 of 21 PC tissues (85.7%), but not in normal pancreatic tissues by immunoblotting and immunohistochemistry. Antisense oligo against ROCK-1 significantly inhibited the haptotaxis of Panc-1 in a dose-dependent manner, compared with the control oligo. Conclusion: These results suggest that ROCK-1 may contribute to pancreatic cancer cell invasion and/or metastasis by facilitating cancer cell migration.
AB - Introduction: Invasion and metastasis of cancer cells require cell motility and adhesion. The small GTPase Rho and one of its effector molecules ROCK regulate cytoskeleton and actomyosin contractility, and play a crucial role in cell adhesion and motility. Results of previous studies showed that the elevated activity of ROCK-1, one of the isomers of ROCK kinases, led to an increase in the activity of invasion and metastasis of cancer cell lines. Aim: To investigate the importance of ROCK-1 in cancer invasion and metastasis. Methodology: We investigated the expression of ROCK-1 in two cancer cell lines and 31 human pancreatic tissues (21 pancreatic cancers [PC] and 10 histologically normal tissues) by immunoblotting and immunohistochemistry. We also examined by haptotaxis assay whether the migratory activity of PC cells could be suppressed by treatment with the morpholino antisense oligonucleotide in vitro. Results: The expression of ROCK-1 was found in 18 of 21 PC tissues (85.7%), but not in normal pancreatic tissues by immunoblotting and immunohistochemistry. Antisense oligo against ROCK-1 significantly inhibited the haptotaxis of Panc-1 in a dose-dependent manner, compared with the control oligo. Conclusion: These results suggest that ROCK-1 may contribute to pancreatic cancer cell invasion and/or metastasis by facilitating cancer cell migration.
KW - Antisense oligonucleotide
KW - Invasion
KW - Metastasis
KW - Pancreatic cancer
KW - Rho-associated kinase
KW - ROCK
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U2 - 10.1097/00006676-200204000-00007
DO - 10.1097/00006676-200204000-00007
M3 - Article
C2 - 11893932
AN - SCOPUS:0036211023
SN - 0885-3177
VL - 24
SP - 251
EP - 257
JO - Pancreas
JF - Pancreas
IS - 3
ER -
