TY - JOUR
T1 - Expression of Six Proteins Causes Reprogramming of Porcine Fibroblasts Into Induced Pluripotent Stem Cells With Both Active X Chromosomes
AU - Fukuda, Tomokazu
AU - Tani, Tetsuya
AU - Haraguchi, Seiki
AU - Donai, Kenichiro
AU - Nakajima, Nobuyoshi
AU - Uenishi, Hirohide
AU - Eitsuka, Takahiro
AU - Miyagawa, Makoto
AU - Song, Sanghoun
AU - Onuma, Manabu
AU - Hoshino, Yumi
AU - Sato, Eimei
AU - Honda, Arata
N1 - Funding Information:
We are grateful to Ms. Toshimi Matsumoto (Advanced Genomics Breeding Section, Institute of Crop Science, National Agriculture and Food Research Organization, 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan) for the technical help of the gene expression analysis. We are also grateful to Dr. Hideko Fukuda (National Institute for Environmental Studies, Tsukuba, Japan) and Dr. Nobuo Goto (Emeritus Professor of Kobe University, Japan) for their support and encouragement throughout this study. This work was supported by JSPS KAKENHI Grant Number 23380157.
Publisher Copyright:
© 2016 Wiley Periodicals, Inc.
PY - 2017/3/1
Y1 - 2017/3/1
N2 - In this study, we created porcine-induced pluripotent stem (iPS) cells with the expression of six reprogramming factors (Oct3/4, Klf4, Sox2, c-Myc, Lin28, and Nanog). The resulting cells showed growth dependent on LIF (leukemia inhibitory factor) and expression of multiple stem cell markers. Furthermore, the iPS cells caused teratoma formation with three layers of differentiation and had both active X chromosomes (XaXa). Our iPS cells satisfied the both of important characteristics of stem cells: teratoma formation and activation of both X chromosomes. Injection of these iPS cells into morula stage embryos showed that these cells participate in the early stage of porcine embryogenesis. Furthermore, the RNA-Seq analysis detected that expression levels of endogenous pluripotent related genes, NANOG, SOX2, ZFP42, OCT3/4, ESRRB, and ERAS were much higher in iPS with six factors than that with four reprogramming factors. We can conclude that the expression of six reprogramming factors enables the creation of porcine iPS cells, which is partially close to naive iPS state. J. Cell. Biochem. 118: 537–553, 2017.
AB - In this study, we created porcine-induced pluripotent stem (iPS) cells with the expression of six reprogramming factors (Oct3/4, Klf4, Sox2, c-Myc, Lin28, and Nanog). The resulting cells showed growth dependent on LIF (leukemia inhibitory factor) and expression of multiple stem cell markers. Furthermore, the iPS cells caused teratoma formation with three layers of differentiation and had both active X chromosomes (XaXa). Our iPS cells satisfied the both of important characteristics of stem cells: teratoma formation and activation of both X chromosomes. Injection of these iPS cells into morula stage embryos showed that these cells participate in the early stage of porcine embryogenesis. Furthermore, the RNA-Seq analysis detected that expression levels of endogenous pluripotent related genes, NANOG, SOX2, ZFP42, OCT3/4, ESRRB, and ERAS were much higher in iPS with six factors than that with four reprogramming factors. We can conclude that the expression of six reprogramming factors enables the creation of porcine iPS cells, which is partially close to naive iPS state. J. Cell. Biochem. 118: 537–553, 2017.
KW - EPIGENETIC STATUS
KW - PLURIPOTENT STEM CELL
KW - PORCINE
KW - TRANSCRIPTIONAL FACTORS
KW - X CHROMOSOME
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U2 - 10.1002/jcb.25727
DO - 10.1002/jcb.25727
M3 - Article
C2 - 27608441
AN - SCOPUS:84991735004
SN - 0730-2312
VL - 118
SP - 537
EP - 553
JO - Journal of Cellular Biochemistry
JF - Journal of Cellular Biochemistry
IS - 3
ER -