TY - JOUR
T1 - Expression of the bph genes involved in biphenyl/PCB degradation in Pseudomonas sp. KKS102 induced by the biphenyl degradation intermediate, 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
AU - Ohtsubo, Yoshiyuki
AU - Nagata, Yuji
AU - Kimbara, Kazuhide
AU - Takagi, Masamichi
AU - Ohta, Akinori
N1 - Funding Information:
YO is financially supported by research fellowships of the Japan Society for the Promotion of Science for Young Scientists. This work was supported in part by a Grant-in-Aid from the Ministry of Education, Science, Sports, and Culture of Japan. This work was performed using the facilities of the Biotechnology Research Center, The University of Tokyo.
PY - 2000/10/3
Y1 - 2000/10/3
N2 - The bph genes involved in PCB/biphenyl degradation in Pseudomonas sp. KKS102 are clustered as bphEGFA1A2A3BCDA4R. The bph genes are inducibly expressed in the presence of biphenyl. In order to understand the induction more fully, the inducer of bph gene expression was investigated. To identify the inducer molecule, we constructed four deletion mutants of the structural genes and analyzed the inducibility of the bphE gene in each mutant strain. In the wild-type cell and the bphD deletion mutant, the levels of the bphE transcript were enhanced in the presence of biphenyl. On the other hand, in the bphA, bphB, and bphC deletion mutants, levels of the bphE transcript were not enhanced in the presence of biphenyl. These results demonstrated that the series of reactions catalyzed by biphenyl dioxygenase (BphA), dihydrodiol dehydrogenase (BphB), and 2,3-dihydroxybiphenyl dioxygenase (BphC) are necessary to convert biphenyl to the inducer. It is known that these reactions convert biphenyl to 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA), and it was found that the expression of the bph genes was induced by purified HOPDA. These results clearly indicate that HOPDA is the inducer of the bph genes in KKS102. Copyright (C) 2000 Elsevier Science B.V.
AB - The bph genes involved in PCB/biphenyl degradation in Pseudomonas sp. KKS102 are clustered as bphEGFA1A2A3BCDA4R. The bph genes are inducibly expressed in the presence of biphenyl. In order to understand the induction more fully, the inducer of bph gene expression was investigated. To identify the inducer molecule, we constructed four deletion mutants of the structural genes and analyzed the inducibility of the bphE gene in each mutant strain. In the wild-type cell and the bphD deletion mutant, the levels of the bphE transcript were enhanced in the presence of biphenyl. On the other hand, in the bphA, bphB, and bphC deletion mutants, levels of the bphE transcript were not enhanced in the presence of biphenyl. These results demonstrated that the series of reactions catalyzed by biphenyl dioxygenase (BphA), dihydrodiol dehydrogenase (BphB), and 2,3-dihydroxybiphenyl dioxygenase (BphC) are necessary to convert biphenyl to the inducer. It is known that these reactions convert biphenyl to 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA), and it was found that the expression of the bph genes was induced by purified HOPDA. These results clearly indicate that HOPDA is the inducer of the bph genes in KKS102. Copyright (C) 2000 Elsevier Science B.V.
KW - Aromatic compound
KW - Biodegradation
KW - Inducer
KW - Transcriptional regulation
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U2 - 10.1016/S0378-1119(00)00349-8
DO - 10.1016/S0378-1119(00)00349-8
M3 - Article
C2 - 11054551
AN - SCOPUS:0034601689
SN - 0378-1119
VL - 256
SP - 223
EP - 228
JO - Gene
JF - Gene
IS - 1-2
ER -