TY - JOUR
T1 - Expression of α-tocopherol-associated genes and α-tocopherol accumulation in Japanese black (Wagyu) calves with and without α-tocopherol supplementation
AU - Haga, S.
AU - Nakano, M.
AU - Ishizaki, H.
AU - Roh, S. G.
AU - Katoh, Kazuo
N1 - Funding Information:
1We are grateful to Koji Kimura (Okayama University) and the staff of the Grassland Research Support Center, NARO Institute of Livestock and Grassland Science, for animal supplies and management. We thank Ms. Reiko Kobayashi and Ms. Ayumi Sueda (NARO Institute of Livestock and Grassland Science) for their skilled technical assistance in sampling and measurement. The Research Information Technology Center (Agriculture, Forestry and Fisheries Research, MAFF, Japan) uses SAS Add-In 6.1 for Microsoft Office. This work was supported by JSPS KAKENHI grant numbers 25292158 and 25871102. 2Corresponding author: hagatiku@affrc.go.jp Received March 15, 2015. Accepted June 9, 2015.
Funding Information:
We are grateful to Koji Kimura (Okayama University) and the staff of the Grassland Research Support Center, NARO Institute of Livestock and Grassland Science, for animal supplies and management. We thank Ms. Reiko Kobayashi and Ms. Ayumi Sueda (NARO Institute of Livestock and Grassland Science) for their skilled technical assistance in sampling and measurement. The Research Information Technology Center (Agriculture, Forestry and Fisheries Research, MAFF, Japan) uses SAS Add-In 6.1 for Microsoft Office. This work was supported by JSPS KAKENHI grant numbers 25292158 and 25871102.
Publisher Copyright:
© 2015 American Society of Animal Science. All rights reserved.
PY - 2015/8/6
Y1 - 2015/8/6
N2 - The aim of the study was to clarify 1) the distribution of 6 α-tocopherol (α-Toc)-associated gene expressions in 20 major tissues, including metabolic, reproductive, endocrine, immune, and digestive and absorptive tissues, in relation to α-Toc status and 2) the change in expression patterns of the genes induced when α-Toc was orally administered to Japanese Black (JB) calves. This study examined weaned male JB calves (n = 10), of which 5 calves were orally administered α-Toc for 2 wk (30 IU∙kg-1 BW∙d-1; TOC group). The others did not receive the α-Toc supplement and were the control (CONT) group. The 20 tissues and venous blood (serum) were sampled on the final day. In both groups, the mean mRNA expression levels for α-Toc transfer protein, afamin (AFM), ATP-binding cassette transporter A1, and tocopherol-associated protein were greatest in the liver (P < 0.05), whereas scavenger receptor class B, Type I (SR-BI) mRNA was greatest in the adrenal gland (P < 0.05). The gene for cytochrome P450 family 4, subfamily F, polypeptide 2 was most highly expressed in the liver, testes, and adrenal gland. The α-Toc content was greatest (P < 0.05) in the testes of the 20 sampled tissues in the CONT group. However, the levels in the testes and jejunum were similar and greater (P < 0.05) than the levels in the other 18 tissues in the TOC group. The mean increase in α-Toc levels after oral α-Toc administration (mean α-Toc content for the TOC group divided by the CONT group content) were greater (P < 0.05) in the jejunum (40.7-fold) and duodenum and liver (26.3-and 23.1-fold) than in the serum (7.8-fold). In the liver, α-Toc administration significantly increased (P < 0.05) the AFM and SR-BI mRNA expression levels. The results show that the liver may play an important role in the regulation of α-Toc disposition, but other peripheral tissues that accumulate large amounts of α-Toc could moderate the local α-Toc status and functions, as inferred from the high expressions of the α-Toc-associated genes in JB calves.
AB - The aim of the study was to clarify 1) the distribution of 6 α-tocopherol (α-Toc)-associated gene expressions in 20 major tissues, including metabolic, reproductive, endocrine, immune, and digestive and absorptive tissues, in relation to α-Toc status and 2) the change in expression patterns of the genes induced when α-Toc was orally administered to Japanese Black (JB) calves. This study examined weaned male JB calves (n = 10), of which 5 calves were orally administered α-Toc for 2 wk (30 IU∙kg-1 BW∙d-1; TOC group). The others did not receive the α-Toc supplement and were the control (CONT) group. The 20 tissues and venous blood (serum) were sampled on the final day. In both groups, the mean mRNA expression levels for α-Toc transfer protein, afamin (AFM), ATP-binding cassette transporter A1, and tocopherol-associated protein were greatest in the liver (P < 0.05), whereas scavenger receptor class B, Type I (SR-BI) mRNA was greatest in the adrenal gland (P < 0.05). The gene for cytochrome P450 family 4, subfamily F, polypeptide 2 was most highly expressed in the liver, testes, and adrenal gland. The α-Toc content was greatest (P < 0.05) in the testes of the 20 sampled tissues in the CONT group. However, the levels in the testes and jejunum were similar and greater (P < 0.05) than the levels in the other 18 tissues in the TOC group. The mean increase in α-Toc levels after oral α-Toc administration (mean α-Toc content for the TOC group divided by the CONT group content) were greater (P < 0.05) in the jejunum (40.7-fold) and duodenum and liver (26.3-and 23.1-fold) than in the serum (7.8-fold). In the liver, α-Toc administration significantly increased (P < 0.05) the AFM and SR-BI mRNA expression levels. The results show that the liver may play an important role in the regulation of α-Toc disposition, but other peripheral tissues that accumulate large amounts of α-Toc could moderate the local α-Toc status and functions, as inferred from the high expressions of the α-Toc-associated genes in JB calves.
KW - Cattle
KW - Vitamin E
KW - mRNA expression
KW - α-tocopherol binding protein
UR - http://www.scopus.com/inward/record.url?scp=84975880639&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84975880639&partnerID=8YFLogxK
U2 - 10.2527/jas.2015-9106
DO - 10.2527/jas.2015-9106
M3 - Article
C2 - 26440184
AN - SCOPUS:84975880639
SN - 0021-8812
VL - 93
SP - 4048
EP - 4057
JO - Journal of Animal Science
JF - Journal of Animal Science
IS - 8
ER -