Expression profiling of ETO2-regulated miRNAs in erythroid cells: Possible influence on miRNA abundance

Tohru Fujiwara; Yoko Okitsu; Yuna Katsuoka; Noriko Fukuhara; Yasushi Onishi; Kenichi Ishizawa; Hideo Harigae

doi:10.1016/j.fob.2013.10.004

Expression profiling of ETO2-regulated miRNAs in erythroid cells: Possible influence on miRNA abundance

Tohru Fujiwara, Yoko Okitsu, Yuna Katsuoka, Noriko Fukuhara, Yasushi Onishi, Kenichi Ishizawa, Hideo Harigae

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

ETO2 is a component of a protein complex containing master regulators of hematopoiesis, including GATA-1 and SCL/TAL1, and also has RNA binding properties. Although ETO2 has been reported to repress GATA-1 target genes through histone deacetylation of the target gene loci in erythroid cells, little is known about the contribution of ETO2 to microRNA (miRNA) regulation. Here, we conducted miRNA profiling in ETO2-overexpressing and ETO2-silenced K562 cells. The analysis suggests that ETO2 positively regulates the abundance of mature miRNAs, including miR-21, miR-29b and let-7e. Our data suggest a novel mode of ETO2-mediated target gene repression via effects on miRNA expression.

Original language	English
Pages (from-to)	428-432
Number of pages	5
Journal	FEBS Open Bio
Volume	3
DOIs	https://doi.org/10.1016/j.fob.2013.10.004
Publication status	Published - 2013

Keywords

ETO2
Erythropoiesis
MiRNA

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/j.fob.2013.10.004

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@article{54fdc892d0e14275b90f1f195f85d101,

title = "Expression profiling of ETO2-regulated miRNAs in erythroid cells: Possible influence on miRNA abundance",

abstract = "ETO2 is a component of a protein complex containing master regulators of hematopoiesis, including GATA-1 and SCL/TAL1, and also has RNA binding properties. Although ETO2 has been reported to repress GATA-1 target genes through histone deacetylation of the target gene loci in erythroid cells, little is known about the contribution of ETO2 to microRNA (miRNA) regulation. Here, we conducted miRNA profiling in ETO2-overexpressing and ETO2-silenced K562 cells. The analysis suggests that ETO2 positively regulates the abundance of mature miRNAs, including miR-21, miR-29b and let-7e. Our data suggest a novel mode of ETO2-mediated target gene repression via effects on miRNA expression.",

keywords = "ETO2, Erythropoiesis, MiRNA",

author = "Tohru Fujiwara and Yoko Okitsu and Yuna Katsuoka and Noriko Fukuhara and Yasushi Onishi and Kenichi Ishizawa and Hideo Harigae",

note = "Funding Information: We thank the members of the Hematology and Rheumatology department of Tohoku University Graduate School of Medicine for helpful comments. This study was supported by a Grant-in-Aid from the Ministry of Education, Science, and Culture of Japan (T.F. and H.H.). We also acknowledge the support of Biomedical Research Core of Tohoku University School of Medicine. EPO was a kind gift from Kyowa Hakko Kirin Co. Ltd. (Tokyo, Japan). ",

year = "2013",

doi = "10.1016/j.fob.2013.10.004",

language = "English",

volume = "3",

pages = "428--432",

journal = "FEBS Open Bio",

issn = "2211-5463",

publisher = "Elsevier BV",

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TY - JOUR

T1 - Expression profiling of ETO2-regulated miRNAs in erythroid cells

T2 - Possible influence on miRNA abundance

AU - Fujiwara, Tohru

AU - Okitsu, Yoko

AU - Katsuoka, Yuna

AU - Fukuhara, Noriko

AU - Onishi, Yasushi

AU - Ishizawa, Kenichi

AU - Harigae, Hideo

N1 - Funding Information: We thank the members of the Hematology and Rheumatology department of Tohoku University Graduate School of Medicine for helpful comments. This study was supported by a Grant-in-Aid from the Ministry of Education, Science, and Culture of Japan (T.F. and H.H.). We also acknowledge the support of Biomedical Research Core of Tohoku University School of Medicine. EPO was a kind gift from Kyowa Hakko Kirin Co. Ltd. (Tokyo, Japan).

PY - 2013

Y1 - 2013

N2 - ETO2 is a component of a protein complex containing master regulators of hematopoiesis, including GATA-1 and SCL/TAL1, and also has RNA binding properties. Although ETO2 has been reported to repress GATA-1 target genes through histone deacetylation of the target gene loci in erythroid cells, little is known about the contribution of ETO2 to microRNA (miRNA) regulation. Here, we conducted miRNA profiling in ETO2-overexpressing and ETO2-silenced K562 cells. The analysis suggests that ETO2 positively regulates the abundance of mature miRNAs, including miR-21, miR-29b and let-7e. Our data suggest a novel mode of ETO2-mediated target gene repression via effects on miRNA expression.

AB - ETO2 is a component of a protein complex containing master regulators of hematopoiesis, including GATA-1 and SCL/TAL1, and also has RNA binding properties. Although ETO2 has been reported to repress GATA-1 target genes through histone deacetylation of the target gene loci in erythroid cells, little is known about the contribution of ETO2 to microRNA (miRNA) regulation. Here, we conducted miRNA profiling in ETO2-overexpressing and ETO2-silenced K562 cells. The analysis suggests that ETO2 positively regulates the abundance of mature miRNAs, including miR-21, miR-29b and let-7e. Our data suggest a novel mode of ETO2-mediated target gene repression via effects on miRNA expression.

KW - ETO2

KW - Erythropoiesis

KW - MiRNA

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JO - FEBS Open Bio

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Expression profiling of ETO2-regulated miRNAs in erythroid cells: Possible influence on miRNA abundance

Abstract

Keywords

ASJC Scopus subject areas

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