@article{3004f50c489848eba4e21cf80f144046,
title = "Extended proliferation of chicken- and Okinawa rail-derived fibroblasts by expression of cell cycle regulators",
abstract = "Although immortalized cultured cells are useful for various functional assays or transcriptome analysis, highly efficient and reproducible immortalization methods have not been developed in avian-derived cells. We introduced the simian virus 40 T antigen (SV40T) and human papillomavirus (HPV)-E6E7 to chick and Okinawa rail (endangered species)derived fibroblast. As a result, neither the SV40T nor E6E7 genes could induce avian cell immortality. Accordingly, we attempted to use a recently developed immortalization method, which involved the coexpression of mutant cyclin-dependent kinase 4 (CDK4), Cyclin D, and TERT (K4DT method) in these avian cells. Although the K4DT method could not efficiently induce the efficient immortalization in mass cell population, cellular division until the senescence was significantly extended by K4DT, we succeeded to obtain the immortalized avian cells (chick K4DT: one clone, Okinawa rail K4DT: three clones, Okinawa rail K4DT + telomerase RNA component: one clone) with K4DT expression. We conclude that K4DT expression is used to extend the cell division and immortalization of avian-derived cells.",
keywords = "avian, cell cycle, cellular senescence, endangered animal, immortalized cell",
author = "Masafumi Katayama and Tohru Kiyono and Hitomi Ohmaki and Takahiro Eitsuka and Daiji Endoh and Miho Inoue-Murayama and Nobuyoshi Nakajima and Manabu Onuma and Tomokazu Fukuda",
note = "Funding Information: The authors thank Prof. Akinori Morita (Tokushima University) for helpful suggestion about the p53 inhibitor. The authors are also grateful to Dr. Hiroyuki Miyoshi (Riken BioResources Center, present affiliation: Keio University) for providing the lentivirus plasmids, and its packaging system. They are also gratefully acknowledge helpful discussions with Prof. Katsuhiko Nishimori, Dr. Sizu Hidema, Dr. Kengo Kuroda, and Dr. Kenichiro Donai (Tohoku University). We thank all members of Conservation & Animal Welfare Trust, Yambaru Wildlife Conservation Center and the team of time capsule in National Institute of Environmental Studies for obtaining the Okinawa rail‐derived cells. This study was supported by JSPS KAKENHI (Grant‐in‐Aid for Research Activity start‐up, 16H07432). Kyoto University Supporting program for interaction‐based initiative team studies (SPIRITS) to M.I.‐M. Funding Information: The authors thank Prof. Akinori Morita (Tokushima University) for helpful suggestion about the p53 inhibitor. The authors are also grateful to Dr. Hiroyuki Miyoshi (Riken BioResources Center, present affiliation: Keio University) for providing the lentivirus plasmids, and its packaging system. They are also gratefully acknowledge helpful discussions with Prof. Katsuhiko Nishimori, Dr. Sizu Hidema, Dr. Kengo Kuroda, and Dr. Kenichiro Donai (Tohoku University). We thank all members of Conservation & Animal Welfare Trust, Yambaru Wildlife Conservation Center and the team of time capsule in National Institute of Environmental Studies for obtaining the Okinawa rail-derived cells. This study was supported by JSPS KAKENHI (Grant-in-Aid for Research Activity start-up, 16H07432). Kyoto University Supporting program for interaction-based initiative team studies (SPIRITS) to M.I.-M. Publisher Copyright: {\textcopyright} 2018 Wiley Periodicals, Inc.",
year = "2019",
month = may,
doi = "10.1002/jcp.27417",
language = "English",
volume = "234",
pages = "6709--6720",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "5",
}