Extraction of nobiletin from Citrus Unshiu peels by supercritical fluid and its CRE-mediated transcriptional activity

Chisato Oba; Masaki Ota; Koichiro Nomura; Hironori Fujiwara; Jiro Takito; Yoshiyuki Sato; Yasushi Ohizumi; Hiroshi Inomata

doi:10.1016/j.phymed.2017.01.014

Extraction of nobiletin from Citrus Unshiu peels by supercritical fluid and its CRE-mediated transcriptional activity

Chisato Oba, Masaki Ota, Koichiro Nomura, Hironori Fujiwara, Jiro Takito, Yoshiyuki Sato, Yasushi Ohizumi, Hiroshi Inomata

Tohoku University

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Background Polymethoxyflavone (PMF) is one of bioactive compounds in Citrus Unshiu and included mainly in the peels rather than the fruits, seeds and leaves. Hypothesis/Purpose Supercritical CO₂ extraction is one candidate for selective extraction of polymethoxyflavone and in this study, supercritical CO₂ extraction with/without ethanol entrainer from Citrus Unshiu peels was examined at a temperature of 333 K and a pressure of 30 MPa. Methods CRE (cyclic AMP response element)-mediated transcriptional assay was examined by using the extracts from supercritical fluid extraction. Results The results showed that extracts including nobiletin increased with increasing ethanol concentration in supercritical CO₂ and the elapsed extraction time. Extracts at ethanol concentration of 5 mol% showed high CRE-mediated transcription activity. This can be caused by activity of the extract including nobiletin in addition to the other methoxylated flavonoid species such as tangeretin. Extracts at ethanol concentration of 50% showed the highest CRE-mediated transcription activity, which can be attributed to flavonoid glycoside such as hesperidin. From our investigations, flavonoid glycoside can be one of promoters of CRE-mediated transcription activity.

Original language	English
Pages (from-to)	33-38
Number of pages	6
Journal	Phytomedicine
Volume	27
DOIs	https://doi.org/10.1016/j.phymed.2017.01.014
Publication status	Published - 2017 Apr 15

Keywords

CO
Pharmacological activity
Supercritical fluid extraction

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10.1016/j.phymed.2017.01.014

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title = "Extraction of nobiletin from Citrus Unshiu peels by supercritical fluid and its CRE-mediated transcriptional activity",

abstract = "Background Polymethoxyflavone (PMF) is one of bioactive compounds in Citrus Unshiu and included mainly in the peels rather than the fruits, seeds and leaves. Hypothesis/Purpose Supercritical CO2 extraction is one candidate for selective extraction of polymethoxyflavone and in this study, supercritical CO2 extraction with/without ethanol entrainer from Citrus Unshiu peels was examined at a temperature of 333 K and a pressure of 30 MPa. Methods CRE (cyclic AMP response element)-mediated transcriptional assay was examined by using the extracts from supercritical fluid extraction. Results The results showed that extracts including nobiletin increased with increasing ethanol concentration in supercritical CO2 and the elapsed extraction time. Extracts at ethanol concentration of 5 mol% showed high CRE-mediated transcription activity. This can be caused by activity of the extract including nobiletin in addition to the other methoxylated flavonoid species such as tangeretin. Extracts at ethanol concentration of 50% showed the highest CRE-mediated transcription activity, which can be attributed to flavonoid glycoside such as hesperidin. From our investigations, flavonoid glycoside can be one of promoters of CRE-mediated transcription activity.",

keywords = "CO, Pharmacological activity, Supercritical fluid extraction",

author = "Chisato Oba and Masaki Ota and Koichiro Nomura and Hironori Fujiwara and Jiro Takito and Yoshiyuki Sato and Yasushi Ohizumi and Hiroshi Inomata",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier GmbH",

year = "2017",

month = apr,

day = "15",

doi = "10.1016/j.phymed.2017.01.014",

language = "English",

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pages = "33--38",

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T1 - Extraction of nobiletin from Citrus Unshiu peels by supercritical fluid and its CRE-mediated transcriptional activity

AU - Oba, Chisato

AU - Ota, Masaki

AU - Nomura, Koichiro

AU - Fujiwara, Hironori

AU - Takito, Jiro

AU - Sato, Yoshiyuki

AU - Ohizumi, Yasushi

AU - Inomata, Hiroshi

PY - 2017/4/15

Y1 - 2017/4/15

N2 - Background Polymethoxyflavone (PMF) is one of bioactive compounds in Citrus Unshiu and included mainly in the peels rather than the fruits, seeds and leaves. Hypothesis/Purpose Supercritical CO2 extraction is one candidate for selective extraction of polymethoxyflavone and in this study, supercritical CO2 extraction with/without ethanol entrainer from Citrus Unshiu peels was examined at a temperature of 333 K and a pressure of 30 MPa. Methods CRE (cyclic AMP response element)-mediated transcriptional assay was examined by using the extracts from supercritical fluid extraction. Results The results showed that extracts including nobiletin increased with increasing ethanol concentration in supercritical CO2 and the elapsed extraction time. Extracts at ethanol concentration of 5 mol% showed high CRE-mediated transcription activity. This can be caused by activity of the extract including nobiletin in addition to the other methoxylated flavonoid species such as tangeretin. Extracts at ethanol concentration of 50% showed the highest CRE-mediated transcription activity, which can be attributed to flavonoid glycoside such as hesperidin. From our investigations, flavonoid glycoside can be one of promoters of CRE-mediated transcription activity.

AB - Background Polymethoxyflavone (PMF) is one of bioactive compounds in Citrus Unshiu and included mainly in the peels rather than the fruits, seeds and leaves. Hypothesis/Purpose Supercritical CO2 extraction is one candidate for selective extraction of polymethoxyflavone and in this study, supercritical CO2 extraction with/without ethanol entrainer from Citrus Unshiu peels was examined at a temperature of 333 K and a pressure of 30 MPa. Methods CRE (cyclic AMP response element)-mediated transcriptional assay was examined by using the extracts from supercritical fluid extraction. Results The results showed that extracts including nobiletin increased with increasing ethanol concentration in supercritical CO2 and the elapsed extraction time. Extracts at ethanol concentration of 5 mol% showed high CRE-mediated transcription activity. This can be caused by activity of the extract including nobiletin in addition to the other methoxylated flavonoid species such as tangeretin. Extracts at ethanol concentration of 50% showed the highest CRE-mediated transcription activity, which can be attributed to flavonoid glycoside such as hesperidin. From our investigations, flavonoid glycoside can be one of promoters of CRE-mediated transcription activity.

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KW - Pharmacological activity

KW - Supercritical fluid extraction

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Extraction of nobiletin from Citrus Unshiu peels by supercritical fluid and its CRE-mediated transcriptional activity

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