Fabrication of an electrochemical sensor array for 2D H₂O ₂ imaging

To achieve accurate real-time 2D H₂O₂ distribution imaging, we fabricated an H₂O₂ sensor array by dispensing small quantities of enzyme solution very accurately onto electrodes in an electrode array. We confirmed the accuracy of the dispensing technique. We then investigated the responses of each sensor to H₂O₂ injection using a 64-channel multipotentiostat and found they varied by 20%. Real-time 2D H₂O₂ imaging was therefore successfully realized by using our flow cell system. This sensor array will be useful for the non-invasive, real-time monitoring of the H₂O₂ distribution in biological samples.