Fabrication of microbial chip using collagen gel microstructure

Takatoshi Kaya, Kuniaki Nagamine, Daisuke Oyamatsu, Hitoshi Shiku, Matsuhiko Nishizawa, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


A microbial chip was fabricated by filling the micropores on a glass substrate with collagen-embedded Escherichia coli (E. coli) cells, and characterized by scanning electrochemical microscopy (SECM) in a solution containing ferricyanide. The activity of the E. coli cells in the collagen gel microstructure was imaged and characterized with SECM by mapping the localized concentration of ferrocyanide produced by the respiration of the cells. The SECM-based activity measurement detected as low as approximately 100 E. coli cells. Furthermore, the optical-microscopic observation indicated that the E. coli cells on the chip proliferated during the incubation. The sequential SECM measurements were performed for the same E. coli chip to obtain the microbial growth curve for a small number of microorganisms.

Original languageEnglish
Pages (from-to)313-317
Number of pages5
JournalLab on a Chip
Issue number4
Publication statusPublished - 2003 Nov


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