TY - JOUR
T1 - Fast varifocal two-photon microendoscope for imaging neuronal activity in the deep brain
AU - Sato, Masaaki
AU - Motegi, Yuki
AU - Yagi, Shogo
AU - Gengyo-Ando, Keiko
AU - Ohkura, Masamichi
AU - Nakai, Junichi
N1 - Funding Information:
This work was supported by the program for Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) and the Japan Agency for Medical Research and Development (AMED), and by KAKENHI Grants 15H05723 and 16H06536 from MEXT and the Japan Society for the Promotion of Science (JSPS) to J.N. and MEXT/JSPS KAKENHI Grants 16K13109 and 17H05985 to M.S.
Publisher Copyright:
© 2017 Optical Society of America.
PY - 2017/9/1
Y1 - 2017/9/1
N2 - Fluorescence microendoscopy is becoming a promising approach for deep brain imaging, but the current technology for visualizing neurons on a single focal plane limits the experimental efficiency and the pursuit of three-dimensional functional neural circuit architectures. Here we present a novel fast varifocal two-photon microendoscope system equipped with a gradient refractive index (GRIN) lens and an electrically tunable lens (ETL). This microendoscope enables quasi-simultaneous imaging of the neuronal network activity of deep brain areas at multiple focal planes separated by 85-120 µm at a fast scan rate of 7.5-15 frames per second per plane, as demonstrated in calcium imaging of the mouse dorsal CA1 hippocampus and amygdala in vivo.
AB - Fluorescence microendoscopy is becoming a promising approach for deep brain imaging, but the current technology for visualizing neurons on a single focal plane limits the experimental efficiency and the pursuit of three-dimensional functional neural circuit architectures. Here we present a novel fast varifocal two-photon microendoscope system equipped with a gradient refractive index (GRIN) lens and an electrically tunable lens (ETL). This microendoscope enables quasi-simultaneous imaging of the neuronal network activity of deep brain areas at multiple focal planes separated by 85-120 µm at a fast scan rate of 7.5-15 frames per second per plane, as demonstrated in calcium imaging of the mouse dorsal CA1 hippocampus and amygdala in vivo.
KW - Endoscopic imaging
KW - Fluorescence microscopy
KW - Gradient-index lenses
KW - Nonlinear microscopy
KW - Three-dimensional microscopy
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U2 - 10.1364/BOE.8.004049
DO - 10.1364/BOE.8.004049
M3 - Article
AN - SCOPUS:85028753431
SN - 2156-7085
VL - 8
SP - 4049
EP - 4060
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 9
M1 - #297011
ER -