Fibroblast growth factor-2 regulates the synthesis of hyaluronan by human periodontal ligament cells

Yoshio Shimabukuro, Tomoo Ichikawa, Shinichi Takayama, Satoru Yamada, Masahide Takedachi, Mami Terakura, Tomoko Hashikawa, Shinya Murakami

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)


Basic fibroblast growth factor (FGF-2) can enhance biological potentials of periodontal ligament cells and its topical application induces considerable periodontal tissue regeneration in vivo. In this study, we examined the effect of FGF-2 on the production of hyaluronan (HA), an extracellular matrix playing important roles in homeostasis and inflammatory/wound healing responses, by human periodontal ligament (HPDL) cells. An inhibition binding-protein assay revealed that FGF-2 significantly increased HA production by HPDL cells in a dose dependent manner. Analysis by HPLC revealed that in conditioned medium of FGF-2-treated HPDL cells HA had a higher molecular mass, compared to that of untreated HPDL cells. RT-PCR analysis revealed the enhancement of mRNA expression of hyaluronan synthase (HAS) 1 and HAS 2, both of which contribute to the production of HA with a high molecular mass, but not HAS 3 in the FGF-2-treated HPDL cells. In contrast, three isoforms of hyaluronidase (HYAL) transcript were unchanged in the FGF-2-treated HPDL cells. These results provide new evidence for the possible involvement of FGF-2 in the regulation of HA production and its appreciable roles in not only homeostasis but also regeneration of periodontal tissues.

Original languageEnglish
Pages (from-to)557-563
Number of pages7
JournalJournal of Cellular Physiology
Issue number3
Publication statusPublished - 2005 Jun 1
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology


Dive into the research topics of 'Fibroblast growth factor-2 regulates the synthesis of hyaluronan by human periodontal ligament cells'. Together they form a unique fingerprint.

Cite this