TY - JOUR
T1 - Field performance and cytology of protoplast-derived rice (Oryza sativa)
T2 - high yield and low degree of variation of four japonica cultivars
AU - Ogura, H.
AU - Kyozuka, J.
AU - Hayashi, Y.
AU - Koba, T.
AU - Shimamoto, K.
PY - 1987/9
Y1 - 1987/9
N2 - Protoplast-derived rice plants of four Japanese cultivars, Nipponbare, Fujisaka 5, Norin 14 and Iwaimochi were individually cultivated in a submerged paddy field. They exhibited more stems, which resulted in more panicles than respective control plants. Other characteristics of protoplast-derived plants were (compared with controls): a slightly shorter or similar culm length, fewer spikelets per panicle, slightly lower seed fertility and similar or lighter 1,000 kernel weight. Grain yield of protoplast-derived plants was more than that of respective control plants in four cultivars. The cause of the higher yield of protoplast-derived plants seems to be mainly due to increased panicle number. Among 126 protoplast-derived plants, 1 triploid, 10 tetraploids and 1 aneuploid were found. Furthermore, 11 variants with low seed fertility showing no gross chromosomal anomalies and one plant with abnormal panicles were found. In total, about 80% of protoplastderived plants showed normal characters. The present results are encouraging for the possibility of rice breeding using protoplasts.
AB - Protoplast-derived rice plants of four Japanese cultivars, Nipponbare, Fujisaka 5, Norin 14 and Iwaimochi were individually cultivated in a submerged paddy field. They exhibited more stems, which resulted in more panicles than respective control plants. Other characteristics of protoplast-derived plants were (compared with controls): a slightly shorter or similar culm length, fewer spikelets per panicle, slightly lower seed fertility and similar or lighter 1,000 kernel weight. Grain yield of protoplast-derived plants was more than that of respective control plants in four cultivars. The cause of the higher yield of protoplast-derived plants seems to be mainly due to increased panicle number. Among 126 protoplast-derived plants, 1 triploid, 10 tetraploids and 1 aneuploid were found. Furthermore, 11 variants with low seed fertility showing no gross chromosomal anomalies and one plant with abnormal panicles were found. In total, about 80% of protoplastderived plants showed normal characters. The present results are encouraging for the possibility of rice breeding using protoplasts.
KW - Agronomic characters
KW - Cytology
KW - Protoplast-derived plants
KW - Rice cultivars
KW - Somaclonal variation
UR - http://www.scopus.com/inward/record.url?scp=0003319540&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0003319540&partnerID=8YFLogxK
U2 - 10.1007/BF00288869
DO - 10.1007/BF00288869
M3 - Article
AN - SCOPUS:0003319540
SN - 0040-5752
VL - 74
SP - 670
EP - 676
JO - Theoretical And Applied Genetics
JF - Theoretical And Applied Genetics
IS - 5
ER -