Fish oil changes the lifespan of Caenorhabditis elegans via lipid peroxidation

Soko Sugawara, Taro Honma, Junya Ito, Ryo Kijima, Tsuyoshi Tsuduki

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)


Recently, we administered fish oil containing eicosapentaenoic acid and docosahexaenoic acid (DHA) to senescence-accelerated mice P8 (SAMP8), in order to investigate the effects on lifespan. Surprisingly, the lifespan of SAMP8 that were fed fish oil was shortened significantly, through a mechanism that likely involved lipid peroxidation. In this study, we investigated this phenomenon In further detail. To examine whether this phenomenon occurs only in SAMP8, we investigated the effect of fish oil on the lifespan of another organism species, Caenorhabditis elegans (C. elegans). C. elegans fed fish oil were cultured and the lifespan monitored. As a consequence of the provision of large amounts of fish oil the lifespan of C. elegans was shortened significantly, whereas an appropriate amount of fish oil extended their lifespan significantly. Lipid peroxide levels in C elegans that were fed fish oil increased significantly in a dose-dependent manner. However, lipid peroxide levels in C. elegans were inhibited by the addition of fish oil and an antioxidant, α-tocopherol, and completely abrogated the changes in the lifespan. To further confirm whether the oxidation of n-3 polyunsaturated fatty acid in fish oil would change the lifespan of C. elegans, the effect of oxidized DHA was examined. Large amounts of oxidized DHA were found to shorten their lifespan significantly. Thus, fish oil changes the lifespan of C. elegans through lipid peroxidation.

Original languageEnglish
Pages (from-to)139-145
Number of pages7
JournalJournal of Clinical Biochemistry and Nutrition
Issue number2
Publication statusPublished - 2013 Mar 1


  • C. elegans
  • Fish oil
  • Lifespan
  • Lipid peroxidation
  • N-3 polyunsaturated fatty acid


Dive into the research topics of 'Fish oil changes the lifespan of Caenorhabditis elegans via lipid peroxidation'. Together they form a unique fingerprint.

Cite this