FLAG tagging by CuAAC and nanogram-scale purification of the target protein for a bioactive metabolite involved in circadian rhythmic leaf movement in Leguminosae

Yoshiyuki Manabe, Makoto Mukai, Satoko Ito, Nobuki Kato, Minoru Ueda

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

We report a stepwise FLAG-tagging strategy for the purification of target proteins for bioactive metabolites. This method realizes the microscale purification and identification of target protein from as few as 1 × 105 differentiated cells. Using this method, we isolated and identified MetE as a cytosolic target protein of potassium isolespedezate, a metabolite controlling plant nyctinasty.

Original languageEnglish
Pages (from-to)469-471
Number of pages3
JournalChemical Communications
Volume46
Issue number3
DOIs
Publication statusPublished - 2010

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